Cell Culture Engineering

Recombinant Protein Production

Gyun Min Lee, Helene Faustrup Kildegaard, Sang Yup Lee, Jens Nielsen, Gregory Stephanopoulos

• Publisher: Wiley-VCH
• Year: 2019
• Print ISBN: 9783527343348
• eBook ISBN: 9783527811403
• Edition: 1
• Topic: Biological Sciences
• Subtopic: Biotechnology
• Index: Biological Sciences

Offers a comprehensive overview of cell culture engineering, providing insight into cell engineering, systems biology approaches and processing technology

In Cell Culture Engineering: Recombinant Protein Production, editors Gyun Min Lee and Helene Faustrup Kildegaard assemble top class authors to present expert coverage of topics such as: cell line development for therapeutic protein production; development of a transient gene expression upstream platform; and CHO synthetic biology. They provide readers with everything they need to know about enhancing product and bioprocess attributes using genome-scale models of CHO metabolism; omics data and mammalian systems biotechnology; perfusion culture; and much more.

This all-new, up-to-date reference covers all of the important aspects of cell culture engineering, including cell engineering, system biology approaches, and processing technology. It describes the challenges in cell line development and cell engineering, e.g. via gene editing tools like CRISPR/Cas9 and with the aim to engineer glycosylation patterns. Furthermore, it gives an overview about synthetic biology approaches applied to cell culture engineering and elaborates the use of CHO cells as common cell line for protein production. In addition, the book discusses the most important aspects of production processes, including cell culture media, batch, fed-batch, and perfusion processes as well as process analytical technology, quality by design, and scale down models.


-Covers key elements of cell culture engineering applied to the production of recombinant proteins for therapeutic use
-Focuses on mammalian and animal cells to help highlight synthetic and systems biology approaches to cell culture engineering, exemplified by the widely used CHO cell line
-Part of the renowned "Advanced Biotechnology" book series

Cell Culture Engineering: Recombinant Protein Production will appeal to biotechnologists, bioengineers, life scientists, chemical engineers, and PhD students in the life sciences.

Table of contents

• Cover
• Table of Contents
• About the Series Editors
• 1 Platform Technology for Therapeutic Protein Production
• 2 Cell Line Development for Therapeutic Protein Production
• 3 Transient Gene Expression‐Based Protein Production in Recombinant Mammalian Cells
• 4 Enhancing Product and Bioprocess Attributes Using Genome‐Scale Models of CHO Metabolism
• 5 Genome Variation, the Epigenome and Cellular Phenotypes
• 6 Adaption of Generic Metabolic Models to Specific Cell Lines for Improved Modeling of Biopharmaceutical Production and Prediction of Processes
• 7 Toward Integrated Multi‐omics Analysis for Improving CHO Cell Bioprocessing
• 8CRISPR Toolbox for Mammalian Cell Engineering
• 9CHO Cell Engineering for Improved Process Performance and Product Quality
• 10 Metabolite Profiling of Mammalian Cells
• 11 Current Considerations and Future Advances in Chemically Defined Medium Development for the Production of Protein Therapeutics in CHO Cells
• 12 Host Cell Proteins During Biomanufacturing
• 13 Mammalian Fed‐batch Cell Culture for Biopharmaceuticals
• 14 Continuous Biomanufacturing
• 15 Process Analytical Technology and Quality by Design for Animal Cell Culture
• 16 Development and Qualification of a Cell Culture Scale‐Down Model
• Index
• End User License Agreement