- 197 pages
- English
- PDF
- Available on iOS & Android
eBook - PDF
Introduction to Experimental Biophysics - A Laboratory Guide
About this book
Easily Get Started with Biological ExperimentsIntroduction to Experimental Biophysics - A Laboratory Guide presents wet lab methods for courses in biophysics or molecular biology. A companion to the author's highly praised An Introduction to Experimental Biophysics: Biological Methods for Physical Scientists, this manual offers a flexible course pl
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Yes, you can access Introduction to Experimental Biophysics - A Laboratory Guide by Jay L. Nadeau in PDF and/or ePUB format, as well as other popular books in Medicine & Biochemistry in Medicine. We have over one million books available in our catalogue for you to explore.
Information
Table of contents
- Front Cover
- Contents
- Acknowledgment
- About the Authorďżź
- Preface: How to Use This Manual
- Introduction: Infrastructure and Safety Requirements
- 1âTransformation of Competent Bacteria with a DNA Plasmid
- 2âIsolation of Plasmids by Miniprep and Restriction Enzyme Analysis
- 3âDesigning a New Construct Using Restriction Enzymes (Computer Lab)
- 4âRestriction Enzymes
- 5âLigation
- 6âScreening a Ligation and Choosing Recombinants
- 7âUse of Software to Design Primers for Cloning Using the Polymerase Chain Reaction (Computer Lab)
- 8âRunning the Polymerase Chain Reaction
- 9âProtein Expression and Purification
- 10âCharacterization of Purified Protein
- 11âProtein Crystallization
- 12âTransfecting Mammalian Cells with Custom-ÂDesigned Constructs
- 13âScreening Fluorescent Reporter Gene Expression in Mammalian Cells
- 14âSynthesis of Quantum Dots
- 15âSynthesis of Gold Nanoparticles
- 16âElectrophysiology: Lipid Bilayer Recording
- 17âElectrophysiology Alternative: Introduction to Lipid Vesicles
- Appendix 1âDisposables and Supplies Needed
- Appendix 2âInstructorsâ Guide to Behind-Âthe-ÂScenes Preparation
- Appendix 3âGrading Recommendations and Guidelines
- Appendix 4âAdvanced Options: Customizing the Course
- Back Cover