Sedimentation Velocity Analytical Ultracentrifugation
eBook - PDF

Sedimentation Velocity Analytical Ultracentrifugation

Discrete Species and Size-Distributions of Macromolecules and Particles

  1. 244 pages
  2. English
  3. PDF
  4. Available on iOS & Android
eBook - PDF

Sedimentation Velocity Analytical Ultracentrifugation

Discrete Species and Size-Distributions of Macromolecules and Particles

About this book

A follow-up to the experimental and instrumental aspects described in Basic Principles of Analytical Ultracentrifugation, the volume Sedimentation Velocity Analytical Ultracentrifugation: Discrete Species and Size-Distributions of Macromolecules and Particles describes the theory and practice of data analysis. Mathematical models for the sedimentation process and the evolution of detected signals are developed in a comprehensive framework, jointly with the description of current and historical strategies for how to extract from noisy experimental data the physical parameters of interest, such as size, mass, and shape, composition, and polydispersity of sedimenting particles.

The methods are extensively illustrated, and supported with practical applications, as well as cross-references where to find the methods in the public domain software SEDFIT and SEDPHAT. The systems covered are discrete or polydisperse mixtures of sedimenting molecules or particles in dilute solution, such as proteins and other biomolecules and their stable complexes, man-made polymers, and nanoparticles, observed in different optical systems. A useful reference for researchers and graduate students of macromolecular disciplines, these methods form the essential foundation for the analysis of dynamic interacting systems, which are covered in the volume Sedimentation Velocity Analytical Ultracentrifugation: Interacting Systems.

Software referenced in the book is available for download at: https://sedfitsedphat.nibib.nih.gov/default.aspx

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Yes, you can access Sedimentation Velocity Analytical Ultracentrifugation by Peter Schuck in PDF and/or ePUB format, as well as other popular books in Medicine & Biochemistry in Medicine. We have over one million books available in our catalogue for you to explore.

Information

Publisher
CRC Press
Year
2016
eBook ISBN
9781498768955

Table of contents

  1. Cover
  2. Half Title
  3. Title Page
  4. Copyright Page
  5. Dedication
  6. Table of Contents
  7. Foreword
  8. Preface
  9. Chapter 1: Basic Analysis Principles
  10. Chapter 2: Sedimentation of Discrete Non-Interacting Particles
  11. Chapter 3: Properties of Sedimentation Coefficient Distributions
  12. Chapter 4: Distributions of Non-Diffusing Particles
  13. Chapter 5: Distributions of Diffusing Particles
  14. Chapter 6: Sedimentation Coefficient Distributions from Boundary Derivatives and Extrapolations
  15. Chapter 7: Multi-Component Distributions
  16. Chapter 8: Practical Analysis of Non-Interacting Systems
  17. Appendix A: Numerical Solutions of the Lamm Equation
  18. Appendix B: Calculating Distributions
  19. Bibliography
  20. Index