Protocols used in Molecular Biology
Sandeep Singh, Dhiraj Kumar
- English
- ePUB (mobile friendly)
- Available on iOS & Android
Protocols used in Molecular Biology
Sandeep Singh, Dhiraj Kumar
About This Book
Protocols used in Molecular Biology is a compilation of several examples of molecular biology protocols. Each example is presented with a concise introduction, materials and chemicals required, a step-by-step procedure and troubleshooting tips. Information about the applications of the protocols is also provided. The techniques included in this book are essential to research in the fields of proteomics, genomics, cell culture, epigenetic modification and structural biology. The protocols can also be used by clinical researchers (neuroscientists and oncologists, for example) for medical applications (diagnostics, therapeutics and multidisciplinary projects). Techniques explained in this reference include: - Nucleic Acid (DNA/RNA) isolation - Next-generation sequencing through real time PCR - Western Blotting - 2-D gel electrophoresis - Immunohistochemistry - Chromatin immunoprecipitation (ChIP) - Live cell-culture techniques - Golgi Staining Key Features: - Recent laboratory protocols - Diverse examples of molecular biology experiments - Simple step-by-step presentation of information - Special focus on scientific and clinical applications Protocols used in Molecular Biology is essential reading for academicians, molecular biologists, as well as graduate and undergraduate students studying basic and applied research. Pharmacologists, and medical researchers can also benefit from the wide array of techniques presented in the book.
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Table of contents
- Welcome
- Table of Content
- Title
- BENTHAM SCIENCE PUBLISHERS LTD.
- FOREWORD
- PREFACE
- List of Contributors
- Isolation of Genomic DNA From Plant Tissues
- RNA Isolation Protocol from Cells and Tissues
- Analyzing Gene Expression through Real Time PCR while Neo-tissue Regeneration using Deve- loped Tissue Constructs
- A Modified Western Blot Protocol for Enhanced Sensitivity in the Detection of a Tissue Protein
- Immunohistochemistry as an Important Technique in Experimental and Clinical Practices
- Protocols for the Detection and Proteome Analysis of the Yellow Mosaic Virus Infected Soyabean Leaves
- 2D-DIGE A Powerful Tool for Proteome Analysis
- Molecular Techniques for Genotyping
- Sodium Bisulfite Conversion of Human Genome for DNA Methylation Studies
- Chromatin Immunoprecipitation (ChIP)
- Osteosarcoma Cell Culture and Maintenance to Detect the Apoptotic Effect of Some Promising Compounds by Potent Markers viz. DNA Fragmentation and Caspase-3 Activation
- Culture and Maintenance of Human Ovarian Carcinoma Cells for Scrutinizing Anti-cancerous Activities of Various Compounds via Some Potent Molecular Markers
- Dictyostelium Discoideum: Live Cell Imaging in Changing Perspective
- The Recent Advancement in Rapid Golgi Method and Result Interpretation
- Molecular Markers for the Evaluation of Clonal Fidelity in Medicinal Plants