A Practical Guide to Protein and Peptide Purification for Microsequencing
eBook - ePub

A Practical Guide to Protein and Peptide Purification for Microsequencing

  1. 184 pages
  2. English
  3. ePUB (mobile friendly)
  4. Available on iOS & Android
eBook - ePub

A Practical Guide to Protein and Peptide Purification for Microsequencing

About this book

Why a Second Edition?The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry.- Mass spectrophotometric characterization of proteins and peptides- N-terminal sequencing of proteins with blocked N termini- Internal amino acid sequence analysis after protease digestion in-gel and on-blot- Improved microscale peptide purification methods- Computer analysis of protein sequences- New protocols tested and refined through everyday use in authors' laboratories- Updated reference chapter covering all aspects of protein microsequencing

Frequently asked questions

Yes, you can cancel anytime from the Subscription tab in your account settings on the Perlego website. Your subscription will stay active until the end of your current billing period. Learn how to cancel your subscription.
At the moment all of our mobile-responsive ePub books are available to download via the app. Most of our PDFs are also available to download and we're working on making the final remaining ones downloadable now. Learn more here.
Perlego offers two plans: Essential and Complete
  • Essential is ideal for learners and professionals who enjoy exploring a wide range of subjects. Access the Essential Library with 800,000+ trusted titles and best-sellers across business, personal growth, and the humanities. Includes unlimited reading time and Standard Read Aloud voice.
  • Complete: Perfect for advanced learners and researchers needing full, unrestricted access. Unlock 1.4M+ books across hundreds of subjects, including academic and specialized titles. The Complete Plan also includes advanced features like Premium Read Aloud and Research Assistant.
Both plans are available with monthly, semester, or annual billing cycles.
We are an online textbook subscription service, where you can get access to an entire online library for less than the price of a single book per month. With over 1 million books across 1000+ topics, we’ve got you covered! Learn more here.
Look out for the read-aloud symbol on your next book to see if you can listen to it. The read-aloud tool reads text aloud for you, highlighting the text as it is being read. You can pause it, speed it up and slow it down. Learn more here.
Yes! You can use the Perlego app on both iOS or Android devices to read anytime, anywhere — even offline. Perfect for commutes or when you’re on the go.
Please note we cannot support devices running on iOS 13 and Android 7 or earlier. Learn more about using the app.
Yes, you can access A Practical Guide to Protein and Peptide Purification for Microsequencing by Paul T. Matsudaira in PDF and/or ePUB format, as well as other popular books in Biological Sciences & Biochemistry. We have over one million books available in our catalogue for you to explore.

Information

Publisher
Academic Press
Year
2012
Print ISBN
9780124802827
eBook ISBN
9780080924618
Edition
2
Topic
Biological Sciences
Subtopic
Biochemistry
Index
Biological Sciences

Table of contents

  1. Cover image
  2. Title page
  3. Table of Contents
  4. Copyright
  5. Contributors
  6. Preface
  7. Preface to the First Edition
  8. Abbreviations
  9. Introduction
  10. Chapter 1: Strategies for Obtaining Partial Amino Acid Sequence Data from Small Quantities (<500 pmol) of Pure or Partially Purified Protein
  11. Chapter 2: Enzymatic Digestion of Proteins and HPLC Peptide Isolation
  12. Chapter 3: Purification of Proteins and Peptides by SDS–PAGE
  13. Chapter 4: Internal Amino Acid Sequence Analysis of Proteins after in Situ Protease Digestion on Nitrocellulose
  14. Chapter 5: Mass Spectrometric Strategies for the Structural Characterization of Proteins
  15. SEQUENCES OF COMMONLY USED PROTEASES
  16. References