eBook - ePub
A Practical Guide to Protein and Peptide Purification for Microsequencing
Paul T. Matsudaira, Paul T. Matsudaira
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- 184 pages
- English
- ePUB (mobile friendly)
- Available on iOS & Android
eBook - ePub
A Practical Guide to Protein and Peptide Purification for Microsequencing
Paul T. Matsudaira, Paul T. Matsudaira
Book details
Table of contents
Citations
About This Book
Why a Second Edition?
The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry.
- Mass spectrophotometric characterization of proteins and peptides
- N-terminal sequencing of proteins with blocked N termini
- Internal amino acid sequence analysis after protease digestion in-gel and on-blot
- Improved microscale peptide purification methods
- Computer analysis of protein sequences
- New protocols tested and refined through everyday use in authors' laboratories
- Updated reference chapter covering all aspects of protein microsequencing
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