A Practical Guide to Protein and Peptide Purification for Microsequencing
eBook - ePub

A Practical Guide to Protein and Peptide Purification for Microsequencing

Paul T. Matsudaira, Paul T. Matsudaira

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  1. 184 pages
  2. English
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  4. Available on iOS & Android
eBook - ePub

A Practical Guide to Protein and Peptide Purification for Microsequencing

Paul T. Matsudaira, Paul T. Matsudaira

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About This Book

Why a Second Edition?
The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry.

  • Mass spectrophotometric characterization of proteins and peptides
  • N-terminal sequencing of proteins with blocked N termini
  • Internal amino acid sequence analysis after protease digestion in-gel and on-blot
  • Improved microscale peptide purification methods
  • Computer analysis of protein sequences
  • New protocols tested and refined through everyday use in authors' laboratories
  • Updated reference chapter covering all aspects of protein microsequencing

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Information

Year
2012
ISBN
9780080924618
Subtopic
Biochimica
Edition
2

Table of contents