
- 290 pages
- English
- PDF
- Available on iOS & Android
Techniques in Free Radical Research
About this book
Free radical species are generally short-lived due to their high reactivity and thus direct measurement and identification are often impossible. ESR is the only technique which has the potential for direct detection of radicals but in biological systems even these must be trapped by a spin-trapping agent. Thus most investigations involve recognition of indicators of the presence of radicals in vivo or "FOOTPRINTS" of radical-mediated damage.Techniques in Free Radical Research assembles and critically assesses the most relevant and reliable experimental approaches used towards the measurement of radicals and radical-mediated damage in chemical systems, in cells and in tissues under the following six headings: a) Footprints of DNA damage, b) Footprints of protein damage, c) Footprints of lipid peroxidation, d) Footprints of antioxidant consumption, e) Footprints via indirect radical assays, and f) Footprints via the availability of transition metal complexes.
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Table of contents
- Front Cover
- Techniques in Free Radical Research, Volume 22
- Copyright Page⤀
- Contents
- Preface
- Chapter 1. Introduction to free radicals
- Chapter 2. Mechanisms of radical production
- Chapter 3. The detection and characterization of free radical species
- Chapter 4. Transition metal complexes as sources of radicals
- Chapter 5. Investigation of the consequences of free radical attack on lipids
- Chapter 6. Assay of antioxidant nutrients and antioxidant enzymes
- Chapter 7. Detection of protein structural modifications induced by free radicals
- Chapter 8. Determination of radical damage to DNA
- Appendix B– Methods for isolation of low density lipoproteins and erythrocyte membranes and cytoskeleton from blood
- References
- Subject Index