Microbial Interactions at Nanobiotechnology Interfaces

Molecular Mechanisms and Applications

R. Navanietha Krishnaraj, Rajesh K. Sani, R. Navanietha Krishnaraj, Rajesh K. Sani

• Publisher: Wiley
• Year: 2021
• ISBN:
  9781119617174
• Edition: 1
• Topic: Technology & Engineering
• Subtopic: Nanotechnology & MEMS
• Index: Technology & Engineering

MICROBIAL INTERACTIONS AT NANOBIOTECHNOLOGY INTERFACES

This book covers a wide range of topics including synthesis of nanomaterials with specific size, shape, and properties, structure-function relationships, tailoring the surface of nanomaterials for improving the properties, interaction of nanomaterials with proteins/microorganism/eukaryotic cells, and applications in different sectors.

This book also provides a strong foundation for researchers who are interested to venture into developing functionalized nanomaterials for any biological applications in their research. Practical concepts such as modelling nanomaterials, and simulating the molecular interactions with biomolecules, transcriptomic or genomic approaches, advanced imaging techniques to investigate the functionalization of nanomaterials/interaction of nanomaterials with biomolecules and microorganisms are some of the chapters that offer significant benefits to the researchers.

1
Shape‐ and Size‐Dependent Antibacterial Activity of Nanomaterials

Senthilguru Kulanthaivel and Prashant Mishra

Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, New Delhi, India

Objectives

• To understand the importance of studying nanomaterials‐bio interface and the factors that dictate it.
• To understand the current situation of antibiotic resistance of microbes and their effect on worldwide healthcare.
• To know the strategies to overcome the antibiotic resistance mechanism and role of nanomaterials in combatting it.
• To understand the mechanism of action of antimicrobial nanomaterials and the factors that influence their antimicrobial properties.
• To understand the key role of size and shape of the nanomaterials on the antimicrobial properties of nanomaterials.

1.1 Introduction

Over the past three decades “Nanotechnology” has emerged as a promising strategy to overcome impasses that have accumulated in various fields of science and technology (Albanese, Tang, & Chan, 2012). Nanomaterials (NMs) are defined as minuscule structures having at least one of their dimensions equal to or between 1 and 100 nm. Since there is no single universally accepted definition, so far various organizations have given their own definition to the term “NMs” (Boverhof et al., 2015). US Food and Drug Administration (USFDA) defines nanomaterial as “materials that have at least one dimension in the range of approximately 1–100 nm and exhibit dimension dependent phenomena” (Bleeker et al., 2012). As per Environmental Protection Agency (EPA) definition, “NMs can exhibit unique properties dissimilar than the equivalent chemical compound in a larger dimension.” Similarly, the International Organization for Standardization (ISO) defines it as “material with any external nanoscale dimension or having internal nanoscale surface structure.” EU Commission has described it as “a manufactured or natural material that possesses unbound, aggregated or agglomerated particles where external dimensions are between 1–100 nm size range” (Jeevanandam et al., 2018).

NMs generally exist in the shape of spheres, cubes, rods, tubes, flowers, and platelets (Machado et al., 2015). NMs in the nanoscale dimensions possess a high surface area‐to‐volume ratio and also a high number of atoms/molecules present on the surface rather than the interior of the materials. These are the properties that majorly contribute to the unique functionality of the nanoscale materials, which vary from the bulk of the same material. Therefore, modulation in their structural properties such as change of size or shape will significantly affect their optical, electrical, magnetic, and biological activity (He et al., 2010; Machado et al., 2015). This is one of the distinct advantages of nanotechnology where by engineering the design or production parameters we can modulate the functionality of the NMs specific to particular application (Machado et al., 2015). Hence, the recent studies in nanotechnology are majorly focused on understanding the effect of physicochemical properties such as size, shape, and surface chemistry of the material on the optical, electrical, magnetic, and biological activities.

Considering the aforementioned advantages, NMs have found enormous applications in various fields and products such as cosmetics, catalysts, fillers, biomedical devices, and semiconductors. As per data report from 2015, approximately more than 1800 products from 622 companies in 32 countries contain engineered NMs. In summary, 762 (i.e. around 42%) of the total products are used in the health and fitness category where silver is the most predominantly used NM, in almost 435 products, which are around 24% of the total. Further, about 528 products (i.e. 29% of total) contain NMs as liquid suspension where dermal contact is highly possible. Hence, the abundant application of these materials is leading towards a long‐term co‐existence of such NMs with living systems which may result in adverse toxicological effects to the living bodies. In this context, it is necessary to study the effect of these materials on biological entities such as proteins, DNA, RNA, cell membranes, cell organelles, cells, tissues, and organs. The interactions between the biological system and NMs strongly depend upon the environment and the biophysico‐chemical property of the nano‐bio interface (Nel et al., 2009). As discussed, size, shape, and surface chemistry are the most important factors that govern the physicochemical properties of the NM that in turn throws light at the nano‐bio interface. Understanding the effect of these physicochemical factors and extrapolating them toward the interactions at the nano‐bio interface would help us to design or engineer NMs for specific applications with an added advantage of minimal toxicity to living bodies. There are a number of analytical tools to study the interaction of nano‐biomolecules/proteins. Among them the most employed are mass spectroscopy, Fourier transform infrared spectroscopy, circular dichroism spectroscopy, Raman spectroscopy, nuclear magnetic resonance, UV–vis spectroscopy, surface plasma resonance, quartz crystal balance, atomic force microscopy, fluorescence correlation spectroscopy, fluorescence spectroscopy, and isothermal calorimetry (Saptarshi, Duschl, & Lopata, 2013). Among the different strategies, mass spectroscopy‐based proteomics is the most preferred. Even though the technique is a qualitative measure of proteins bound to NMs, such as nanoparticles (NPs), it can be applied over a wide range of NMs. UV–vis spectroscopy is employed to measure changes observed in the adsorption spectrum caused by NM–protein interaction. Similar to UV–vis spectroscopy, fluorescence spectroscopy is employed to measure changes in the fluorescence spectra caused by the binding of protein on to NMs. Surface plasma resonance is used to study changes in electrons' oscillation on the surface of metal NMs as a result of protein interaction with NM. Isothermal calorimetry analysis is employed to determine the binding constant and other thermodynamic parameters of the nano/bio interface (Saptarshi et al., 2013). Quartz crystal balance is used to measure changes in mass on the surface of oscillating quartz caused by the NM–protein interaction. In a study, adsorption of proteins myoglobin, bovine serum albumin, and cytochrome over the surface of gold NPs was studied using quartz crystal balance (Kaufman et al., 2007). Confocal Raman spectroscopy and confocal spectroscopy can be employed to study and visualize NM–protein interaction and intake of NMs into cells by fluorescent labeling of NPs. In recent times, a combination of these techniques has been strategically employed to study the different aspects of NM–protein/bi...