• The supplementation of prill fat and prill fat along with rumen protected choline chloride did not affect feed intake. • Supplementation of prill fat (2.5% of DMI) and prill fat along with rumen protected choline chloride (54g/animal/d) in early lactating Murrah buffaloes significantly increased the milk yield and milk fat up to 12.10 and 21.76% and 6.34 and 11.3%, respectively, over the control group.• Blood triglyceride (mg/dl) level was significantly (p<0.01) increased whereas NEFA (mmol/l) level was significantly reduced in protected choline + prill fat supplemented group followed by prill fat supplemented group as compare to control group. Other blood metabolites were not affected due to supplementation.• Addition of prill fat and prill fat along with rumen protected choline chloride resulted in improved body condition score and added income of Rs.7.14 and 18.76 per animal per day, respectively, in Murrah buffaloes. The study revealed that supplementing prill fat in the ration of Murrah buffaloes helped in improving milk yield and fat per cent, which can be further enhanced by supplementing the ration with rumen protected choline chloride.