Urinary tract infection or UTI is very common but can be unpleasant and very uncomfortable. Urinary tract infection is an infection in any part of urinary system like kidney, bladder and urethra. Hibiscus is a flowering plant which grows in many tropical places all around world. It is simply known as a beautiful flower however, this plant particularly its leaves and flowers is used in Indian Ayurvedic system as a medicinal plant for treating ailments and health problems. It is used in skin care products for protecting against ultraviolet radiations, kidney disease, hypertension control, cancer treatment, wound healer, reducing cholesterol level, hair care, tumor fighter and laxative. It is also used in many health drinks as a medicinal tea using the flowers and leaves. This study isolated and identified the pathogenic bacteria from urine sample of UTI patient and detected the antibiotic susceptibility against the bacterial isolate and compared the best antibiotic concentration with Hibiscus leaf extract against the test isolate Escherichia coli.