- 144 pages
- English
- ePUB (mobile friendly)
- Available on iOS & Android
Stepwise Culture of Human Adult Stem Cells
About this book
This volume on stepwise techniques to culture adult human stem cells begins with the basic methods to culture adult stem cells followed by related methods to supplement stem cell culture. This and future volumes of techniques will allow laboratories across scientific entities – academic and commercial – to adapt the described methods for similar methods with cells from other species. The standardized techniques will allow across the board interpretation of other technologies such as Omics studies. These methods can be a starting platform for culturing stem cells even in laboratories that might not be familiar with the techniques.
Scientific and early clinical trials strongly favor the development of translational studies with adult stem cells. A major impediment to progress in the field of regenerative medicine is a lack of standardized methods to culture specific stem cells. Such documented methods would allow for scientific rigor and reproducibility to enhance efficient and safe translation of stem cells to patients. This book fills that gap.
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Information
Table of contents
- Cover Page
- Half Title page
- Series Page
- Title Page
- Copyright Page
- Contents
- Preface
- List of Contributors
- List of Figures
- List of Abbreviations
- Introduction: Core Techniques in Stem Cell Culture
- 1 Isolation of Hematopoietic Stem Cells
- 2 Isolation Protocol for CD133 + and CD34 + Very Small Embryonic-like Cells (VSEL) from Human Umbilical Cord Blood
- 3 Isolation and Expansion of Mesenchymal Stem Cells from the Adult Bone Marrow
- 4 Manual Isolation and Expansion of Adiposederived Stem Cells from Adipose Tissue
- 5 Isolating Compact Bone-derived Mesenchymal Stem Cells from Rodent and Rabbit Femurs
- 6 Isolating Dental Pulp Stem Cells
- 7 A Murine System for Somatic Cellular Reprogramming into Induced Pluripotent Stem Cells
- 8 Isolation and Expansion of Placental Stem Cells (P-MSCs)
- 9 Expanding Human Mesenchymal Stem Cells from the Umbilical Cord
- 10 Laboratory Isolation of Leukemia Stem Cells
- 11 Isolation and Characterization of Cancer Stem Cells from Glioblastoma Multiforme and Breast Cancer Cell Lines
- 12 Bone Marrow Aspirate for Isolation of Mesenchymal Stem Cells
- 13 Culturing Human Bone Marrow Stromal Cells
- 14 Isolating Mononuclear Cells by Ficoll-Hypaque Density Gradient
- 15 Phenotypic and Multipotent Characterization of Bone-Marrow-derived Mesenchymal Stem Cells
- 16 Cryopreservation of Stem Cells
- 17 Cryopreservation of Mobilized Peripheral Blood Stem Cells
- 18 Manual Cell Count
- Index