Laboratory Practices in Microbiology

Osman Erkmen

• Publisher: Academic Press
• Year: 2021
• Print ISBN: 9780323910170
• eBook ISBN: 9780323900157
• Topic: Biological Sciences
• Subtopic: Microbiology
• Index: Biological Sciences

Laboratory Practices in Microbiology provides updated insights on methods of isolation and cultivation, morphology of microorganisms, the determination of biochemical activities of microorganisms, and physical and chemical effects on microorganisms. Sections cover methods of preparation of media and their sterilization, microorganisms in environment, aseptic techniques, pure culture techniques, preservation of cultures, morphological characteristics of microorganisms, wet-mount and hanging-drop techniques, different staining techniques, cultural and biochemical characteristics of bacteria, antimicrobial effects of agents on microorganisms, hand scrubbing in the removal of microorganisms, characteristics of fungi, uses of bacteriophages in different applications, and more. Applications are designed to be common, complete with equipment, minimal expense and quick to the markets. Images are added to applications, helping readers better follow the expressions and make them more understandable. This is an essential book for students and researchers in microbiology, the health sciences, food engineering and technology, and medicine, as well as anyone working in a laboratory setting with microorganisms. - Gives complete explanations for all steps in experiments, thus helping readers easily understand experimental procedures - Includes certain subjects that tend to be disregarded in other microbiology laboratory books, including microorganisms in the environment, pure culture methods, wet-mount and hanging drop methods, biochemical characteristics of microorganisms, osmotic pressure effects on microorganisms, antiseptic and disinfectants effects on microorganisms, and more - Provides groupings and characterizations of microorganisms - Functions as a representative reference book for the field of microbiology in the laboratory

Table of contents

• Laboratory Practices in Microbiology
• Practice 1 Preparation of media and sterilization techniques
• Practice 2 Microorganisms in environment
• Practice 3 Aseptic techniques
• Practice 4 Pure culture techniques
• Practice 5 Culture preservation techniques
• Practice 6 Microscope for study of microorganisms
• Practice 7 Microbial cell measurement technique
• Practice 8 Wet-mount and hanging-drop techniques
• Practice 9 Smear preparation and simple staining techniques
• Practice 10 Gram staining technique
• Practice 11 Bacterial endospore staining techniques
• Practice 12 Bacterial capsule staining techniques
• Practice 13 Motility tests
• Practice 14 Cultural characteristics of bacteria
• Practice 15 Oxidation and fermentation
• Practice 16 Hydrolytic reactions
• Practice 17 Antiseptic and disinfectant effects on microorganisms
• Practice 18 Antibiotic sensitivity test technique
• Practice 19 Effect of hand scrubbing on removal of microorganisms
• Practice 20 Temperature effects on microorganisms
• Practice 21 Osmotic pressure and pH effects on microorganisms
• Practice 22 Ultraviolet light effects on microorganisms
• Practice 23 Oxygen effects on microorganisms
• Practice 24 Morphological characteristics of yeasts and molds
• Practice 25 Isolation, enumeration, and uses of bacteriophages
• Appendix Preparation of culture media, stains, and solutions
• Index