The Chlamydomonas Sourcebook

Volume 2: Organellar and Metabolic Processes

Arthur Grossman, Francis-André Wollman

• Publisher: Academic Press
• Year: 2023
• eBook ISBN: 9780323910583
• Edition: 3
• Topic: Biological Sciences
• Subtopic: Biochemistry
• Index: Biological Sciences

Originally published as the stand-alone Chlamydomonas Sourcebook, then expanded as the second volume in a three-part comprehensive gold-standard reference, The Chlamydomonas Sourcebook: Organellar and Metabolic Processes has been fully revised and updated to include a wealth of new knowledge and resources for the Chlamydomonas community. It details the tremendous progress recently made with respect to imaging the ultrastructure of cells, dissecting acclimation and biosynthetic responses, and elucidating molecular processes underlying the biology of organelles. In particular, this volume includes exciting new developments in the use of imaging technologies for examining supramolecular organization of the chloroplast, defining mechanisms of branched electron transfer pathways in photosynthesis, dissecting the organization of pyrenoids and CO2 concentration mechanisms, presenting the intricacies associated with acclimation to environmental conditions and providing new insights into dark metabolism and the network of fermentative metabolism. This book thus presents the latest advances in both the research and uses of new experimental approaches and technologies, making this a must-have resource for researchers and students working in plant science and photosynthesis, fertility, mammalian vision, aspects of human disease, acclimation to environmental change, and the biogenesis of cellular complexes. - Describes molecular techniques, analysis of the recently sequenced genome, reviews of the current status of the diverse fields in which Chlamydomonas is used as a model organism - Provides methods for Chlamydomonas research and best practices for their applications; this includes methods for cell culture, preservation of cultures, preparation of media, lists of inhibitors, and other additives to culture media, classical genetic manipulation, and new approaches for gene transfer and editing technologies - Assists researchers with common laboratory problems such as contamination

Table of contents

• Title of Book
• Cover image
• Title page
• Table of Contents
• Copyright
• List of contributors
• Preface
• Introduction
• Historical overview
• Chapter 1 The comparative, biochemistry, genetics, and evolution of starch metabolism in Chlamydomonas reinhardtii
• Chapter 2 Chlamydomonas glycerolipid metabolism
• Chapter 3 Nitrogen metabolism in Chlamydomonas
• Chapter 4 Phosphorus and sulfur uptake, assimilation, and deprivation responses
• Chapter 5 Trace metal nutrition and response to deficiency
• Chapter 6 Sensory photoreceptors in Chlamydomonas
• Chapter 7 Rubisco and inorganic carbon assimilation
• Chapter 8 Photoproduction of reducing power and the Calvin-Benson cycle
• Chapter 9 Metabolic networks during dark anoxia
• Chapter 10 Hydrogenases and hydrogen production
• Chapter 11 The mitochondrion: from genome to proteome
• Chapter 12 The chloroplast in a changing environment: from genome to proteome
• Chapter 13 Control of organellar gene expression by nucleus-encoded proteins
• Chapter 14 Translation and protein synthesis in the chloroplast
• Chapter 15 Photosynthesis: light harvesting
• Chapter 16 Photosystems I and II
• Chapter 17 Chloroplast ATP synthase and the cytochrome b6f complex
• Chapter 18 The multiple routes of photosynthetic electron transfer in Chlamydomonas reinhardtii
• Chapter 19 The assembly of photosynthetic proteins
• Chapter 20 Molecular chaperones, proteases, and unfolded protein responses
• Chapter 21 Tetrapyrrole biosynthesis and signaling (chlorophyll, heme, and bilins)
• Chapter 22 Carotenoids in Chlamydomonas
• Chapter 23 Supramolecular organization of chloroplast membranes
• Chapter 24 State transitions
• Chapter 25 Photoprotection
• Index