Stem Cell Laboratory Techniques: A Guide for Researchers and Students introduces the reader to stem cell culture, handling techniques and versatile applications used by researchers. Sections introduce stem cells, including definitions, types and basic use of stem cells in biomedical science research. The book explains laboratory procedures and techniques ranging from the extraction of stem cells from animals, cell seeding and culture, harvest and maintenance of stem cells, stem cell characterization, accurate recording, quality control, and more. In addition, it guides researchers on topics such as transcriptome analysis, proliferation study analysis, and microphysiological study. Final sections cover useful and recent applications in stem cells, such as gene editing techniques and the preparation of stem cells for in vitro study, as well as stem cell lab design and equipment used in the lab. Lastly, human and animal research ethics are discussed. - Introduces readers to the stem cell culture and moves to handling techniques and versatile applications - Includes coverage of gene editing techniques for stem cells and stem cells for in vitro study - Presents stem cell lab design and equipment used in the lab

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Yes, you can access Stem Cell Laboratory Techniques by Suresh Kumar Kailasa, Akon Higuchi, Pooi Ling Mok, Suresh Kumar Subbiah,Akon Higuchi,Pooi Ling Mok,Suresh Kumar Kailasa in PDF and/or ePUB format, as well as other popular books in Biological Sciences & Biology. We have over one million books available in our catalogue for you to explore.

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Title of Book
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Chapter 1 Introduction: Stem cells and their application in research and therapy
Chapter 2 Animal stem cells: Extraction, expansion, and cryopreservation
Chapter 3 Protocols in stem cell culture
Chapter 4 Isolation of human stem cells from full-term gestation amniotic fluid using immunoselection and one-stage culture techniques
Chapter 5 Isolation and characterization of c-kit+ stem cells from rat full-term gestation amniotic fluid
Chapter 6 Human induced pluripotent stem cells (hiPSCs): Generation, characterization, and differentiation
Chapter 7 Confirmation of stem cells pluripotency via teratoma formation in an animal model
Chapter 8 Gene editing for stem cells by CRISPR-Cas9
Chapter 9 Hypoxic environment: A new solution to stem cell culture
Chapter 10 Mouse embryonic stem cell-derived cerebral organoids
Chapter 11 Standardized practice of stem cell production
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About this book

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