Recombinant DNA Laboratory Manual
eBook - PDF

Recombinant DNA Laboratory Manual

  1. 208 pages
  2. English
  3. PDF
  4. Available on iOS & Android
eBook - PDF

Recombinant DNA Laboratory Manual

About this book

Recombinant DNA Laboratory Manual is a laboratory manual on the fundamentals of recombinant DNA techniques such as gel electrophoresis, in vivo mutagenesis, restriction mapping, and DNA sequencing. Procedures that are useful for studying either prokaryotes or eukaryotes are discussed, and experiments are included to teach the fundamentals of recombinant DNA technology. Hands-on computer sessions are also included to teach students how to enter and manipulate sequence information. Comprised of nine chapters, this book begins with an introduction to bacterial growth parameters, how to measure bacterial cell growth, and how to plot cell growth data. The discussion then turns to the isolation and analysis of chromosomal DNA in bacteria and Drosophila; plasmid DNA isolation and agarose gel analysis; and introduction of DNA into cells. Subsequent chapters deal with Tn5 mutagenesis of pBR329; DNA cloning in M13; DNA sequencing; and DNA gel blotting, probe preparation, hybridization, and hybrid detection. The book concludes with an analysis of lambda phage manipulations. This manual is intended for advanced undergraduate or beginning graduate students and should also be helpful to established investigators who are changing their research focus.

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Yes, you can access Recombinant DNA Laboratory Manual by Judith W. Zyskind,Sanford I. Bernstein in PDF and/or ePUB format, as well as other popular books in Biological Sciences & Cell Biology. We have over one million books available in our catalogue for you to explore.

Table of contents

  1. Front Cover
  2. Recombinant DNA Laboratory Manual
  3. Copyright Page
  4. Table of Contents
  5. Dedication
  6. PREFACE
  7. SCHEDULE OF LABORATORY EXERCISES
  8. CHAPTER 1. LAB I. BACTERIAL GROWTH PARAMETERS
  9. CHAPTER 2. LAB II. ISOLATION AND ANALYSIS OF BACTERIAL AND DROSOPHILA CHROMOSOMAL DNA
  10. CHAPTER 3. LAB III. PLASMID DNA ISOLATION AND AGAROSE GEL ANALYSIS
  11. CHAPTER 4. Lab IV. INTRODUCTION OF DNA INTO CELLS
  12. CHAPTER 5. LAB V. TN5 MUTAGENESIS OF PBR329
  13. CHAPTER 6. LAB VI. DNA CLONING IN M13
  14. CHAPTER 7. LAB VII. DNA SEQUENCING
  15. CHAPTER 8. LAB VIII DNA GEL BLOTTING, PROBE PREPARATION, HYBRIDIZATION, AND HYBRID DETECTION
  16. CHAPTER 9. LAB IX LAMBDA PHAGE MANIPULATIONS
  17. Appendix 1: USE OF COMPUTERS IN A MOLECULAR BIOLOGY LABORATORY
  18. Appendix 2: SAFETY IN THE RECOMBINANT DNA LABORATORY
  19. Appendix 3: SOLUTIONS
  20. Appendix 4: STRAINS AND DNA
  21. Appendix 5: MOLECULAR BIOLOGY REAGENT SUPPLIERS
  22. Appendix 6: FREQUENTLY USED ENZYMES
  23. Appendix 7: FREQUENTLY USED TECHNIQUES NOT DESCRIBED IN LABS
  24. INDEX