Immunological Methods

Volume IV

Ivan Lefkovits, Benvenuto Pernis

• Publisher: Academic Press
• Year: 2014
• Print ISBN: 9780124427044
• eBook ISBN: 9781483265360
• Topic: Medicine
• Subtopic: Immunology

Immunological Methods, Volume IV provides information pertinent to the methods in immunological research. This book focuses on cells, clones, and cell lines, as well as on their components and secreted products. Organized into 21 chapters, this volume begins with an overview of hybridoma methodology as the most celebrated immunological method. This text then discusses cell fusion, hybridoma technology, and everything related to monoclonal antibodies. Other chapters consider another molecular biology method, which describes the procedure required for establishing a partitioned cDNA-library. This book provides as well a comprehensive analysis of mRNA populations in which every messenger species appears as a distinct element, and so provides accurate answers to questions concerning genetic complexity. The final chapter provides an example of how transgenic mice can be used to study the development of T cell repertoires. This book is a valuable resource for cell biologists, scientists, immunologists, and research workers.

Table of contents

• Front Cover
• Immunological Methods
• Copyright Page
• Table of Contents
• Contributors
• Preface
• Contents of Previous Volumes
• Abbreviations List
• Chapter 1. Solubilization of Cell Surface Membrane-Associated Molecules by Genetic Engineering
• Chapter 2. Methods and Vectors for Positive Selection and Cell-Free Expression of Lymphocyte cDNA Clones
• Chapter 3. Application of Polymerase Chain Reaction in Molecular Immunology
• Chapter 4. Target Detection Assay: A General Method to Determine DNA Binding Sites for Putative DNA-Binding Proteins
• Chapter 5. Analysis of cis- and trans-Acting Factors Regulating Gene Transcription
• Chapter 6. Cell-Free System for Polyadenylation Using Mouse B Cell Extracts
• Chapter 7. In Vitro Transcriptional Rate Assay for Lymphoid Cells
• Chapter 8. System for High-Level Production in Escherichia coli and Rapid Purification of Recombinant Proteins: Application to Epitope Mapping, Preparation of Antibodies, and Structure-Function Analysis
• Chapter 9. Analysis of Two-Dimensional Gel Electrophoretic Protein Patterns upon in Vivo Labeling of Mice
• Chapter 10. Biological Assay of Interleukins
• Chapter 11. Monoclonal Antibodies against Cytokine Receptors
• Chapter 12. Human Monoclonal Antibodies
• Chapter 13. Isolation of Rat Immuno globulin Class Switch Variants from Hybridomas by Sequential Sublining
• Chapter 14. Mapping of T Cell Epitopes in Proteins: A Chemical Approach
• Chapter 15. Plasmodium Falciparum Malaria Parasite Cultures and Their Use in Immunology
• Chapter 16. A Chemotactic Assay Enabling Recovery of Cells after Migration
• Chapter 17. Assay for Quantitation of Cell–Cell Adhesion Using Fluorescent Dyes
• Chapter 18. Analysis of Avian T and B Lymphocytes
• Chapter 19. Isolation of Cells Involved in the Germinal Center Reaction: Germinal Center B Cells and Follicular Dendritic Cells
• Chapter 20. Applications of Fetal Thymus Organ Cultures in Studies of T Cell Development
• Chapter 21. Transgenic Mice for Analysis of T Cell Development
• Index