Methods in Virology

Volume V

Karl Maramorosch, Hilary Koprowski

• Publisher: Academic Press
• Year: 2014
• Print ISBN: 9780124702059
• eBook ISBN: 9781483262239
• Topic: Biological Sciences
• Subtopic: Immunology
• Index: Biological Sciences

Methods in Virology, Volume V focuses on the methods used in virology, including hybridization, gel electrophoresis, freeze-etching technique, and ultracentrifugation. The selection first offers information on the fusion of cells for virus studies and production of cell hybrids; approaches to ultracentrifugation; and polyacrylamide gel electrophoresis of viral RNA. Discussions focus on applications to virological problems and analysis of results; analysis of the distribution of RNA on polyacrylamide gels after electrophoresis; and biological analysis of DNA components. The book then examines the polyacrylamide gel electrophoresis of viral proteins, DNA-RNA and DNA-DNA hybridization in virus research, and techniques of RNA-DNA hybridization in solution for the study of viral transcription. Topics include preparation of nucleic acids, hybridization and elution procedures that minimize RNA degradation, and procedures for acrylamide gel electrophoresis. The text takes a look at freeze-etching technique for the study of virus ultrastructure; procedures to increase virus yield from infected plants; and the immunoperoxidase technique. Concerns include principles of the immunoperoxidase technique, histochemical detection of peroxidase activity, sequence of events in virus infection, and factors affecting virus yield. The selection is a valuable source of data for researchers interested in the methods employed in virology.

Table of contents

• Front Cover
• Methods in Virology
• Copyright Page
• Table of Contents
• List of Contributors
• Preface
• Contents of Other Volumes
• Chapter 1. Fusion of Cells for Virus Studies and Production of Cell Hybrids
• Chapter 2. New Approaches to Ultracentrifugation
• Chapter 3. Small Circular Viral DNA: Preparation and Analysis
• Chapter 4. Polyacrylamide Gel Electrophoresis of Viral RNA
• Chapter 5. Polyacrylamide Gel Electrophoresis of Viral Proteins
• Chapter 6. DNA—RNA and DNA—DNA Hybridization in Virus Research
• Chapter 7. Techniques of RNA—DNA Hybridizationin Solution for the Study of Viral Transcription
• Chapter 8. Methods for the Study of mRNA Synthesis in Bacteriophage-Infected E. coli
• Chapter 9. Reactions of Viruses in Agar Gel
• Chapter 10. Methods for the Study of Virus–Antibody Complexes
• Chapter 11. Techniques of Ferritin- Tagged Antibodies
• Chapter 12. The Immunoperoxidase Technique: Localization of Viral Antigens in Cells
• Chapter 13. Leaf Dip Serology
• Chapter 14. Procedures to Increase Virus Yield from Infected Plants
• Chapter 15. Freeze-Etching Technique for the Study of Virus Ultrastructure
• Author Index
• Subject Index