Immunological Methods
eBook - PDF

Immunological Methods

Volume III

  1. 506 pages
  2. English
  3. PDF
  4. Available on iOS & Android
eBook - PDF

Immunological Methods

Volume III

About this book

Immunological Methods, Volume III provides information pertinent to the fundamental aspects of immunological methods. This book presents recombinant DNA technology as applied to immunology. Organized into 25 chapters, this volume begins with an overview of the major histocompatibility complex. This text then examines the use of monoclonal antibodies in the identification of lymphocyte membrane antigens and explains the use of monoclonal antibodies in histocompatibility typing. Other chapters consider the methods in two-dimensional electrophoretic analysis of proteins and another to lymphokines that support B cells. This book discusses as well the production and expansion of cloned B and T cell lines and hybridomas. The final chapter deals with the sophisticated methodology used with particular animal species, namely, birds, sheep, and amphibians to probe specific immunological questions. This book is a valuable resource for immunologists and investigators with extensive experience in using immunological methods. Protein chemists and biologists will also find this book useful.

Frequently asked questions

Yes, you can cancel anytime from the Subscription tab in your account settings on the Perlego website. Your subscription will stay active until the end of your current billing period. Learn how to cancel your subscription.
No, books cannot be downloaded as external files, such as PDFs, for use outside of Perlego. However, you can download books within the Perlego app for offline reading on mobile or tablet. Learn more here.
Perlego offers two plans: Essential and Complete
  • Essential is ideal for learners and professionals who enjoy exploring a wide range of subjects. Access the Essential Library with 800,000+ trusted titles and best-sellers across business, personal growth, and the humanities. Includes unlimited reading time and Standard Read Aloud voice.
  • Complete: Perfect for advanced learners and researchers needing full, unrestricted access. Unlock 1.4M+ books across hundreds of subjects, including academic and specialized titles. The Complete Plan also includes advanced features like Premium Read Aloud and Research Assistant.
Both plans are available with monthly, semester, or annual billing cycles.
We are an online textbook subscription service, where you can get access to an entire online library for less than the price of a single book per month. With over 1 million books across 1000+ topics, we’ve got you covered! Learn more here.
Look out for the read-aloud symbol on your next book to see if you can listen to it. The read-aloud tool reads text aloud for you, highlighting the text as it is being read. You can pause it, speed it up and slow it down. Learn more here.
Yes! You can use the Perlego app on both iOS or Android devices to read anytime, anywhere — even offline. Perfect for commutes or when you’re on the go.
Please note we cannot support devices running on iOS 13 and Android 7 or earlier. Learn more about using the app.
Yes, you can access Immunological Methods by Ivan Lefkovits,Benvenuto Pernis in PDF and/or ePUB format, as well as other popular books in Biological Sciences & Immunology. We have over one million books available in our catalogue for you to explore.

Information

Publisher
Academic Press
Year
2014
Print ISBN
9780124427037
eBook ISBN
9781483269634
Topic
Biological Sciences
Subtopic
Immunology
Index
Biological Sciences

Table of contents

  1. Front Cover
  2. Immunological Methods
  3. Copyright Page
  4. Table of Contents
  5. Contributors
  6. Preface
  7. Contents of Previous Volumes
  8. Abbreviations List
  9. Chapter 1. Methods in Molecular Immunology: Chromosomal Walking in the Major Histocompatibility Complex
  10. Chapter 2. DNA-Mediated Tranformation of Lymphoid Cells
  11. Chapter 3. Cloning of cDNAs with Vectors Permitting Expression in Host Cells
  12. Chapter 4. Construction of Vectors for Immunoglobulin Reverse Genetics
  13. Chapter 5. Strategies of Oligonucleotide Synthesis
  14. Chapter 6. High-performance Liquid Chromatography of Biologically Active Proteins in the Nanogram (Picomole) Range
  15. Chapter 7. The HPLC of Immunoglobulins
  16. Chapter 8. Preparation of Liposomes Carrying Lymphocyte Membrane Proteins
  17. Chapter 9. Detection of Glycolipid Antigens with Monoclonal Antibodies
  18. Chapter 10. Mapping of Novel B Lymphocyte Differentiation Antigens
  19. Chapter 11. Use of Large-Scale Two-Dimensional ISODALT Gel Electrophoresis System in Immunology
  20. Chapter 12. Tissue Typing Using Biosynthetically Labeled Monoclonal Antibodies
  21. Chapter 13. Assays for Lymphokines Supporting B Cell Growth
  22. Chapter 14. Establishment of Cellular Microenvironments for in Vitro B Cell Progenitor Differentiation
  23. Chapter 15. Development of B Cell Progenitors in Semisolid Agar Cultures
  24. Chapter 16. Isolation of Human Alloreactive T Helper Clones and Their Use as Polyclonal Activators of B Cells
  25. Chapter 17. Cytolytic T Cell Clones and Hybridomas
  26. Chapter 18. Conditions for the Establishment in Vitro of Interleukin 3-Dependent Murine B Cell Precursor Lines
  27. Chapter 19. Assays for the Detection of Rheumatoid Factor-Producing Cells
  28. Chapter 20. Fluorescence Cell Sorter Techniques in Immunology
  29. Chapter 21. Tables for Evaluating Limiting Diluting Experiments
  30. Chapter 22. Development of an Avian Lymphoid Chimera
  31. Chapter 23. Avian Embryos in Immunology
  32. Chapter 24. Sheep as an Experimental Model for Immunology: Immunological Techniques in Vitro and in Vivo
  33. Chapter 25. Methods Used to Study the Immune System of Xenopus (Amphibia, Anura)
  34. Index