Path to Effective Recovering of DNA from Formalin-Fixed Biological Samples in Natural History Collections
eBook - ePub

Path to Effective Recovering of DNA from Formalin-Fixed Biological Samples in Natural History Collections

Workshop Summary

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  1. English
  2. ePUB (mobile friendly)
  3. Available on iOS & Android
eBook - ePub

Path to Effective Recovering of DNA from Formalin-Fixed Biological Samples in Natural History Collections

Workshop Summary

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About this book

Museums catalogue our knowledge of the Earth's biodiversity, and their collections represent many decades of work by experts. Access to DNA sequence information in archival specimens would greatly extend knowledge of the genetic relationships within our biosphere. However, molecular genetic analysis of museum specimens has been slowed by the usual practice of fixation and storage of samples in formalin. Formalin is an environmental toxin and induces genetic and chromosomal alterations to the samples.Few of the many attempts to obtain and sequence DNA from formalin-fixed specimens stored in aqueous formalin or ethanol have been successful. All of the protocols are slow, difficult, and often expensive, and few produce DNA fragments longer than 500 base pairs. Path to Effective Recovering of DNA from Formalin-Fixed Biological Samples in Natural History Collections examines past attempts on DNA recovery from formalin-preserved biological specimens and discusses the research needed to advance the development of similar but more efficient and cost-effective protocols.

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Table of contents

  1. COVER PAGE
  2. THE NATIONAL ACADEMIES
  3. STEERING COMMITTEE FOR THE WORKSHOP ON RECOVERING DNA FROM FORMALIN-FIXED BIOLOGICAL SAMPLES
  4. BOARD ON LIFE SCIENCES
  5. Preface
  6. Acknowledgments
  7. Contents
  8. Introduction
  9. Workshop Proceedings
  10. References
  11. Appendix A Glossary
  12. Appendix B Participant Biographies
  13. Appendix C Agenda