Techniques in Glycobiology

R. Reid Townsend, Arland T. Hotchkiss

• Publisher: CRC Press
• Year: 1997
• eBook ISBN: 9781040211786
• Topic: Physical Sciences
• Subtopic: Analytic Chemistry

This work covers methodologies for plant and animal glycoconjugate analysis. It details mass spectrometry, nuclear magnetic resonance spectroscopy, glycolipids and new physical methods, o-glycosylation characterization, chromophore and fluorophore labelling of oligosaccharides, separations, exoglycosidases and mapping, and plant glycobiology.

Table of contents

• Cover Page
• Halftitle Page
• Title Page
• Copyright Page
• Preface
• Contents
• Contributors
• 1 New Frontiers in Nuclear Magnetic Resonance Spectroscopy Use of a Nano*NMR Probe for the Analysis of Microgram Quantities of Complex Carbohydrates
• 2 Analysis of Anionic Glycoconjugates by Delayed Extraction Matrix-Assisted Laser Desorption Time-of-Flight Mass Spectrometry
• 3 Matrix-Assisted Laser Desorption/Ionization Time-of-Flight
• 4 The Fragmentation Behavior of Glycopeptides Using the Post-Source Decay Technique and Matrix-Assisted Laser Desorption Ionization Mass Spectrometry
• 5 Application of Electrospray Ionization Mass Spectrometry to Unusual Phosphorylated Oligosaccharides of Trypanosoma cruzi
• 6 Analysis of Carbohydrate-Protein Interactions by Mass Spectrometry
• 7 Mycobacterial Lipoarabinomannans
• 8 Microscale Analysis of Glycosphingolipids by Thin-Layer Chromatography Blotting
• 9 High-Performance Liquid Chromatography of Gangliosides Sandro Sonnino, Manuela Valsecchi, Paola Palestini, Marco Nicolini, and Vanna Chigorno University of Milan, Milan, Italy
• 10 A Sensitive Cell Suspension Enzyme-Linked Immunosorbent Assay Measuring the Density of Cell Surface Ganglioside and Carbohydrate Antigens
• 11 Scattering Techniques for the Study of Aggregative Properties of Gangliosides
• 12 Imaging Carbohydrate Polymers with Noncontact Mode Atomic Force Microscopy
• 13 Novel Approach for the Analysis of Interaction Between Sugar Chains and Carbohydrate-Recognizing Molecules Using a Biosensor Based on Surface Plasmon Resonance
• 14 Glycoprotein Detection Using the Light-Addressable Potentiometric Sensor
• 15 Effects of O-Glycosylation on Protein Structure The Role and Characterization of Heavily O-Glycosylated Mucin Domains
• 16 Predicting Mucin-Type O-Glycosylation Sites
• 17 Experimental Approaches to Studying O-Glycosylation In Vivo
• 18 Identifying Sites of Glycosylation in Proteins
• 19 High Energy Collision-Induced Dissociation of O-Linked Glycopeptides
• 20 Analysis of Permethylated Glycoprotein Oligosaccharide Fractions by Gas Chromatography and Gas Chromatography-Mass Spectrometry
• 21 Highly Sensitive Pre-Column Derivatization Procedures for Quantitative Determination of Monosaccharides, Sialic Acids, and Amino Sugar Alcohols of Glycoproteins by Reversed Phase HighPerformance Liquid Chromatography
• 22 Glycan Labeling with the Fluorophores 2-Aminobenzamide and Anthranilic Acid
• 23 Capillary Gel Electrophoresis of 8-Aminopyreiie-3,6,8-trisiilfoiiate-Labeled Oligosaccharides
• 24 Evaluation of Glycosylpyrazole Derivatives in Carbohydrate Analysis
• 25 Discovery and Uses of Novel Glycosidases
• 26 Resolution of Oligosaccharides in Capillary Electrophoresis
• 27 A Comparison of Oligosaccharide Profiling Methods
• 28 High-Performance Liquid Chromatographic Mapping of Oligosaccharides Using High pH Anion-Exchange Chromatography Improvements from Sample Preparation, Reduction, and Fluorometric Detection
• 29 Monosaccharide Analysis of Recombinant Glycoproteins by High pH Anion-Exchange Chromatography/High-Performance Liquid Chromatography Using a Refractive Index Detector
• 31 Characterization of Subnanomolar Amounts of 7V-Glycans by 2-Aminobenzamide Labeling, Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry, and Computer-Assisted Sequence Analysis
• 32 Isolation and Characterization of TV-Linked Oligosaccharides from Yeast Glycoproteins
• 33 High Resolution Electrophoretic Analysis of Carbohydrates Using a DNA Sequencer
• 34 Recent Developments in Starch Structure Analysis Using High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection
• 35 Red Seaweed Galactans
• 36 Some New Methods to Study Plant Polyuronic Acids and Their Esters
• Index