Methods in Protein Biochemistry

Harald Tschesche

• Publisher: De Gruyter
• Year: 2011
• Print ISBN: 9783110252330
• eBook ISBN: 9783110252361
• Edition: 1
• Topic: Biological Sciences
• Subtopic: Biochemistry
• Index: Biological Sciences

This book presents a survey of recent developments in protein biochemistry. Top researchers in the field of protein biochemistry describe modern methods to address the challenges of protein purification by three-phase partitioning, and their folding and degradation by the functions of chaperones. The significance of peptide purity for fibril formation is addressed as well as the use of target oriented peptide arrays in palliative approaches in mucoviszidose. The design and application of protein epitope mimetics just as the structural resolving of the misfolding of various mutant proteins in serpinopathies enlarge our tools in resolving pathophysiological imbalances.

Table of contents

• Preface
• Editor
• List of contributing authors
• Abbreviations
• Acknowledgements
• 1 Three-phase partitioning
• 2 Folding and degradation functions of molecular chaperones
• 3 Membrane protein folding in detergents
• 4 Glycoprotein-folding quality control in the endoplasmic reticulum
• 5 Conformational dynamics in peptides and proteins studied by triplet-triplet energy transfer
• 6 Protein import into the intermembrane space of mitochondria
• 7 On-membrane identification of gel-resolved proteins by matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS)
• 8 Analysis of protein complexes using chemical cross-linking and mass spectrometry
• 9 Single-crystal spectroscopy correlated with X-ray crystallography provides complementary perspectives on macromolecular func ion
• 10 Wide-angle X-ray solution scattering (WAXS)
• 11 Where purity matters: recombinant versus synthetic peptides in beta amyloid formation
• 12 Chemical modification of proteins in living cells
• 13 Proteomics of human bronchoalveolar lavage fluid: discovery of biomarkers of chronic obstructive pulmonary disease (COPD) with difference gel electrophoresis (DIGE) and mass spectrometry (MS)
• 14 Proteomic analysis of Duchenne muscular dystrophy (DMD)
• 15 Target-oriented peptide arrays in a palliative approach to cystic fibrosis (CF)
• 16 Probing protein dynamics in vivo using backbone cyclization: bacterial acyl carrier protein as a case study
• 17 The protein epitope mimetic approach to protein-protein interaction inhibitors
• 18 The structural biology of α1-antitrypsin deficiency and the serpinopathies
• Index