Mass Spectrometry-Based Chemical Proteomics

W. Andy Tao, Ying Zhang, W. Andy Tao, Ying Zhang

• Publisher: Wiley
• Year: 2019
• Print ISBN: 9781118969557
• eBook ISBN: 9781118970201
• Edition: 1
• Topic: Physical Sciences
• Subtopic: Biochemistry in Medicine

PROVIDES STRATEGIES AND CONCEPTS FOR UNDERSTANDING CHEMICAL PROTEOMICS, AND ANALYZING PROTEIN FUNCTIONS, MODIFICATIONS, AND INTERACTIONS—EMPHASIZING MASS SPECTROMETRY THROUGHOUT

Covering mass spectrometry for chemical proteomics, this book helps readers understand analytical strategies behind protein functions, their modifications and interactions, and applications in drug discovery. It provides a basic overview and presents concepts in chemical proteomics through three angles: Strategies, Technical Advances, and Applications. Chapters cover those many technical advances and applications in drug discovery, from target identification to validation and potential treatments.

The first section of Mass Spectrometry-Based Chemical Proteomics starts by reviewing basic methods and recent advances in mass spectrometry for proteomics, including shotgun proteomics, quantitative proteomics, and data analyses. The next section covers a variety of techniques and strategies coupling chemical probes to MS-based proteomics to provide functional insights into the proteome. In the last section, it focuses on using chemical strategies to study protein post-translational modifications and high-order structures.

• Summarizes chemical proteomics, up-to-date concepts, analysis, and target validation
• Covers fundamentals and strategies, including the profiling of enzyme activities and protein-drug interactions
• Explains technical advances in the field and describes on shotgun proteomics, quantitative proteomics, and corresponding methods of software and database usage for proteomics
• Includes a wide variety of applications in drug discovery, from kinase inhibitors and intracellular drug targets to the chemoproteomics analysis of natural products
• Addresses an important tool in small molecule drug discovery, appealing to both academia and the pharmaceutical industry

Mass Spectrometry-Based Chemical Proteomics is an excellent source of information for readers in both academia and industry in a variety of fields, including pharmaceutical sciences, drug discovery, molecular biology, bioinformatics, and analytical sciences.

Table of contents

• Cover
• Table of Contents
• Preface
• 1 Protein Analysis by Shotgun Proteomics1
• 2 Quantitative Proteomics for Analyses of Multiple Samples in Parallel with Chemical Perturbation
• 3 Chemoproteomic Analyses by Activity‐Based Protein Profiling
• 4 Activity‐Based Probes for Profiling Protein Activities
• 5 Chemical Probes for Proteins and Networks
• 6 Probing Biological Activities with Peptide and Peptidomimetic Biosensors
• 7 Chemoselective Tagging to Promote Natural Product Discovery
• 8 Identification and Quantification of Newly Synthesized Proteins Using Mass Spectrometry‐Based Chemical Proteomics
• 9 Tracing Endocytosis by Mass Spectrometry
• 10 Functional Identification of Target by Expression Proteomics (FITExP)
• 11 Target Discovery Using Thermal Proteome Profiling
• 12 Chemical Strategies to Glycoprotein Analysis
• 13 Proteomic Analysis of Protein–Lipid Modifications: Significance and Application
• 14 Site‐Specific Characterization of Asp‐ and Glu‐ADP‐Ribosylation by Quantitative Mass Spectrometry
• 15MS‐Based Hydroxyl Radical Footprinting: Methodology and Application of Fast Photochemical Oxidation of Proteins (FPOP)
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