Neuroimaging techniques
Thanks to the recent advances in neuroscience, we can explore the function and structure of the brain using a variety of different behavioral and brain measures. Consistent with this chapterâs focus on brain-based approaches, here we provide a short overview of four most commonly used measures: the hemodynamic neuroimaging approach of fMRI, the electrophysiological technique of ERP, sMRI for gray matter changes and DTI for tracking white matter fiber structure. Note that other methods, such as the lesion method, magnetoencephalagraphy (MEG), direct cortical brain stimulation, and transcranial magnetic stimulation (TMS), have also been used in L2 research but are beyond the scope of this chapter.
Functional magnetic resonance imaging (fMRI). FMRI involves the use of strong magnetic fields created by the magnetic coils of the MRI machine to measure hemodynamic changes in blood flow, specifically the blood-oxygen-level-dependent (BOLD) signals, a ratio of oxygenated versus deoxygenated hemoglobin in given brain regions. As we engage in cognitive and linguistic behavior, neuronal cells in certain brain regions consume more energy than in others, and the energy is supplied by hemoglobins, the red proteins that transport oxygen through the red blood cells. FMRI captures these dynamic BOLD activities in various parts of the brain, presumably reflecting underlying neuronal activities related to specific processes of cognition. In general, increased BOLD signals reflect increased cognitive activities, and by comparing the different BOLD signals from cognitive tasks versus those from a baseline task (e.g., a task in which the participant stares at a crosshair on a computer screen), fMRI researchers can make inferences about the role that focused brain regions play in a specific task, be it face recognition or language processing. The spatial resolution of fMRI is excellent by todayâs standard, in millimeter range (e.g., an fMRI voxel may be 1â4 cubic mm in size).
Event-related potentials (ERPs). This method measures the brainâs ongoing electrical activities on a millisecond-by-millisecond basis, a time window within which critical cognitive and linguistic processes take place. When raw EEG signals are averaged over multiple trials of a stimulus condition, and when these signals are âtime-lockedâ to stimulus events (e.g., presentation of a visual word), the corresponding âevent-relatedâ potentials that fluctuate in voltage can be extracted and analyzed. These fluctuations are designated as âcomponentsâ, the âbrainwave peaks and valleysâ. ERP components vary in a number of dimensions, including polarity (positive vs. negative), latency (timing), and amplitude (level), along with distribution information (location) of the activation on the scalp. These components are typically labeled according to the latency of the waveformâs peak amplitude; for example, N400 refers to a negative going waveform peaking at about 400ms post-stimulus onset during visual or auditory presentation. Several key components implicated in language processing have been identified in the literature (see Kutas, Federmeier, Staab, & Kluender, 2007 for a review) including N400, indicating lexical semantic integration in sentences, LAN (left anterior negativity, occurring in the same time window as N400), indicating morpho-syntactic analysis, and P600, indicating syntactic analysis and repair (see Key, Dove, & Maguire, 2005 for a summary of major ERP components relevant to cognition).
Structural magnetic resonance imaging (sMRI). It provides information to qualitatively and quantitatively describe the shape, size, and integrity of gray and white matter structures in the brain. Broadly speaking, MRI signal varies across tissue types because gray matter contains more cell bodies (e.g., neurons and glial cells) than white matter, which is primarily composed of long-range nerve fibers (myelinated axons), along with supporting glial cells. GM density or volume, as one of the most common measures of anatomical brain changes, can be calculated via voxel-based morphometry (VBM), an analytic method that extracts GM information from sMRI scans (see Ashburner & Friston, 2000; Mechelli, Price, Friston, & Ashburner, 2005). VBM typically involves the normalization of each brain scan to a standard stereotactic space (e.g., MNI space), delineation of GM versus WM versus cerebrospinal fluid (CSF), and a voxel-by-voxel analysis of the tissue concentration. VBM identifies the local tissue environment after correction for macroscopic anatomical differences across participants. Besides GM density, sMRI scans can also provide information on cortical thickness (CT) (Fischl & Dale, 2000; Im et al., 2008; Lerch & Evans, 2005). Unlike GM density or volume, CT is a direct measure of cortical morphology. In this technique, voxels are first segmented into GM, WM, or CSF. The boundaries between GM and WM, and between GM and CSF are then delineated either manually or through automated procedures. Finally, the thickness between these surfaces is measured using a variety of methods, each determining the distance between nodes on each surface for the entirety of the cortex examined. CT provides sub-millimeter accuracy and takes into account the folding of the cortical surface. Structurally there may be an inverse relationship between CT and GM due to the cortical folding patterns: thicker cortical regions are less convoluted and therefore have less GM density (see Chung, Dalton, Shen, Evans, & Davidson, 2006).
Diffusion tensor imaging (DTI). It is a MRI-based neuroimaging technique which makes it possible to visualize the location, orientation, and anisotropy of the brainâs white matter tracts. DTI examines the diffusion of water molecules in the brain and compares the degree of diffusivity of neurons along the axon, referred to as axial diffusivity (AD) along with the radial diffusivity (RD) that is perpendicular to the axon diameter (Filler, 2009). Another measure, the mean diffusivity (MD), is used to measure diffusion within a voxel, regardless of orientation, and is calculated by averaging the eigenvalues (Alexander, Lee, Lazar, & Field, 2007). Lower MD values often correspond to greater WM integrity. By far the most commonly used value to calculate the magnitude of diffusion is the fractional anisotropy (FA), a normalized standard diffusivity value between 0 and 1 calculated from the eigenvectors of the diffusion tensor (Assaf & Pasternak, 2008). The higher the FA value, the more integrity the WM has (contrasting the interpretation of the MD value). In studies of English and other Western languages, two WM tracts have been most strongly associated with language representation: the left arcuate fasciculus (AF) component of the superior longitudinal fasciculus (SLF) and the left inferior longitudinal fasciculus (ILF). First, the SLF connects a dorsal language network (Hickok & Poeppel, 2004, 2007), and may consist of (1) a direct AF pathway connecting posterior (superior temporal gyrus/Wernickeâs area) and anterior (inferior frontal gyrus/Brocaâs area) language cortical regions and (2) an indirect pathway including the SLF connecting t...