Electrofocusing and Isotachophoresis
eBook - PDF

Electrofocusing and Isotachophoresis

Proceedings of the International Symposium, August 1–4, 1976, Hamburg, Germany

  1. 624 pages
  2. English
  3. PDF
  4. Available on iOS & Android
eBook - PDF

Electrofocusing and Isotachophoresis

Proceedings of the International Symposium, August 1–4, 1976, Hamburg, Germany

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Yes, you can access Electrofocusing and Isotachophoresis by B. J. Radola, D. Graesslin in PDF and/or ePUB format, as well as other popular books in Biological Sciences & Biochemistry. We have over one million books available in our catalogue for you to explore.

Information

Publisher
De Gruyter
Year
2019
Print ISBN
9783110070262
eBook ISBN
9783110873870
Edition
1
Topic
Biological Sciences
Subtopic
Biochemistry
Index
Biological Sciences

Table of contents

  1. Preface
  2. Contents
  3. Contributors
  4. Part I. Isoelectric Focusing
  5. 1. Theoretical and General Aspects
  6. Reminiscences about the Genesis of Isoelectric Focusing and Generalization of the Idea
  7. Stable pH Gradients - A Key Problem in Isoelectric Focusing
  8. Electrofocusing in Buffers: Formation of Natural pH Gradients, Flexibility, Gradient Stability, Relation to Isotachophoresis and Preparative Potential
  9. Comparison of pH Gradients Used for Isoelectric Focusing
  10. On the Temperature Dependence of Isoelectric Points of Proteins with Special Reference to Isoelectric Focusing
  11. 2. Carrier Ampholytes
  12. Carrier Ampholyte Distribution
  13. Characterization of Carrier Ampholytes by New Visualization Reactions and Chromatographic Methods
  14. Synthesis of Carrier Ampholytes for Isoelectric Focusing Containing Sulfonic and Phosphonic Acid Groups Covering a Wide pH Range
  15. 3. Analytical Methodology
  16. Staining of Proteins in Polyacrylamide Gels
  17. Isoelectric Focusing of Complex Protein Mixtures in the Nanogram Range and Enzyme Kinetics of Dehydrogenases Following IEF
  18. Soft Laser Scanning Densitometer Compatible with the High Resolution Obtained by Electrofocusing
  19. Immunocore Electrofocusing: A Separation and Detection Technique Amenable to Scanning Densitometry
  20. Rapid, Convenient and Economical Procedures for the Determination of the Isoelectric Spectra of Proteins
  21. A Simple Method of Choosing Optimum pH-Conditions for Electrophoresis
  22. pH Determinations in Isoelectric Focusing with an Iridium Electrode
  23. Anomalous Behaviour of Horseradish Peroxidase in Isoelectric Focusing
  24. Does Lipoprotein Lipase Bind Ampholytes?
  25. 4. Biochemical Applications
  26. Zein: Macromolecular Properties, Biosynthesis and Genetic Regulation
  27. Growth and Isoelectric Patterns of Peroxidase in Tobacco Tissue Cultures Under the Influence of Growth Regulator Systems
  28. A Method for Simultaneous Analysis of Several Genetic Loci in Mice After Electrofocusing
  29. Phylogenetic Differences in Isoelectric Components of Liver Acid Phosphatase and LDH Between Genera of Two Closely Related Rodent Families (Muridae and Microtidae)
  30. Fractionation of Nucleic Acids on Isoelectric Focusing
  31. 5. Clinical Applications
  32. Polyacrylamide Gel Isoelectric Focusing of Alpha-1-Proteinase Inhibitors (α<sub>1 </sub>Pi) Phenotypes: I. Optimal Condition, Histochemical and Proteinase Probe Studies
  33. Polyacrylamide Gel Isoelectric Focusing (PAGIF) Applied to Alpha-1-Proteinase Inhibitor (α<sub>1 </sub>Pi) Phenotyping: II. Immunochemical Procedures
  34. High Resolution Phenotyping of α<sub>1</sub>-Antitrypsins by Thin-Layer Polyacrylamide Electrofocusing
  35. Broad-Beta Disease (Hyperlipoproteinaemia Type III): Genetics, Gene Frequency and Diagnosis without Ultracentrifugation
  36. Screening for Abnormal Hemoglobins in the Newborn: A Highly Economic Procedure Using Isoelectric Focusing
  37. The Crystallins of the Aging Lens from Five Species Studies by Various Methods of Thin-Layer Isoelectric Focusing
  38. Separation of Erythrocyte Membrane Components in Electric Field. Polyacrylamide Gel Isoelectric Focusing: A Potential Method for the Diagnosis of Membrane Abnormality
  39. Microheterogeneity of Serum Glycoproteins as Revealed by Flat-Bed Gel Isoelectric Focusing
  40. Diagnosis of Cystic Fibrosis (CF) - Purification and Distinction Between CF Protein and Ciliary Dyskinesia Activity in CF and Asthmatic Sera Using Isoelectric Focusing and the Rabbit Tracheal Ciliary Bioassay
  41. Microheterogeneity of Human Kininogen, Alpha- 2-HS and Alpha-1-Acid Glycoproteins by Thin- Layer Isoelectric Focusing
  42. A Genetic Variant of Amylase from Human Parotid Saliva Detected by Isoelectric Focusing
  43. 6. Two-Dimensional Techniques
  44. Biological Applications of Two-Dimensional Gel Electrophoresis
  45. Plant Proteins Evaluated by Two-Dimensional Methods
  46. Two-Dimensional Separation of Lymphocyte Microsomal Membrane Proteins: Isoelectric Focusing Linked to SDS-Polyacrylamide Gel Electrophoresis
  47. Mammalian Mitochondrial Ribosomes: Two- Dimensional Gel Electrophoresis of the 55 S- and the Corresponding Subunit Proteins
  48. Crossed Immunoelectrofocusing for Standardization and Characterization of Fungal Antigens
  49. 7. Preparative Separations
  50. Isoelectric Focusing in Free Water
  51. Preparative Electrofocusing in Flat-Beds of Granulated Gel - Methodological Aspects
  52. Preparative Scale Purification of Bacterial Enzymes and Toxins by Isoelectric Focusing and Isotachophoresis
  53. Preparative Zone Convection Electrofocusing in Helical Glass Tubes
  54. Chromatofocusing: Isoelectric Focusing on Ion Exchangers in the Absence of an Externally Applied Potential
  55. 8. Separation of Cells
  56. New Instrumentation and Procedures for the Analysis of Mammalian Cells by Electrophoretic Techniques
  57. Continuous-Flow Isoelectric Focusing: Further Studies on Human Red Blood Cells and on the Separation of Rat Liver Light Mitochondrial Fraction
  58. Preparative Electrofocusing of Native and Modified Living Mammalian Cells in a Stationary Ficoll/ Sucrose Gradient
  59. Part II. Isotachophoresis
  60. 1. Analytical and Preparative Methodology
  61. The Principle of Preparative Capillary Isotachophoresis
  62. Preparative Capillary Isotachophoresis: Separation of μg Amounts of Some Human Serum Proteins
  63. Quantitative Determination of Picomoles ADP by Means of Steady State Mixed Zones in Isotachophoresis
  64. Thin-Layer Polyacrylamide Gel Isotachophoresis, Crossed Immuno-Isotachophoresis and Crossed Isotachophoresis-Electrofocusing
  65. Isotachophoresis of the Cerebrospinal Fluid (CSF) Proteins: Advantage of Prior Dialysis and its Practical Realization
  66. 2. Applications
  67. Plasma Protein Fractionation in Sephadex by Isotachophoresis Using Discrete Spacers
  68. Immuno-lsoelectric Focusing Analysis of Antibodies Fractionated by Isotachophoresis
  69. Analytical and Preparative Isotachophoresis in Column and Flat-Bed Gels for Fractionation of Antibodies
  70. Index