Principles of Proteomics
eBook - PDF

Principles of Proteomics

Richard Twyman, Ph.D Cfe, George A.

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  1. 260 pages
  2. English
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eBook - PDF

Principles of Proteomics

Richard Twyman, Ph.D Cfe, George A.

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About This Book

Principles of Proteomics, Second Edition, provides a concise and user-friendly introduction to the diverse technologies used for the large-scale analysis of proteins, as well as their applications, and their impact in areas such as drug discovery, agriculture, and the fight against disease. Proteomics is a fast-advancing field in which researchers seek to capture all the proteins in the cell and characterize them in ever more detail. Principles of Proteomics has been fully updated to reflect the most recent developments in the field without losing its focus on the underlying principles. With worked examples, case studies profiling both established and emerging technologies, and further reading lists for each chapter, Principles of Proteomics is an ideal introduction for students, researchers and those working in the industry.

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Information

Publisher
Garland Science
Year
2013
ISBN
9781317753025
Edition
2
Topic
Médecine
Subtopic
Biochimie en médecine
10
CHAPTER 
1: 
THE 
ORIGIN 
AND 
SCOPE 
OF 
PROTEOMICS
and 
color 
plates). 
For 
both 
devices, 
a 
population 
of 
mRNA 
molecules 
from 
a 
particular 
source 
is 
reverse-transcribed 
en 
masse 
to 
form 
a 
representative 
complex 
cDNA 
population. 
In 
the 
case 
of 
spotted 
microarrays, 
a 
fluoro-
phore-conjugated 
nucleotide 
is 
included 
in 
the 
reaction 
mix 
so 
that 
the 
cDNA 
population 
is 
universally 
labeled. 
In 
the 
case 
of 
oligonucleotide 
chips, 
the 
unlabeled 
cDNA 
is 
converted 
into 
a 
labeled 
cRNA 
(complementary 
Spotted 
DNA 
microarray
High-density 
oligonucleotide 
array
Sequence 
selection
(b)
Gene 
X
Hybridize
Hybridize
Wash
Wash
Stain
Scan
Combined 
data 
in 
software
X
Z
Y
Scan 
2
Scan 
1
Image 
2
Image 
1
Image 
2
Image 
1
X
X
Y
Y
Z
Z
Gene 
X
(a)
(d)
(c)
DNA
Public
database
Glass
wafer
PM
MM
RNA 
1
+ 
fluor
Combined
image 
in
software
RNA 
1
+ 
biotin
RNA 
2
+ 
biotin
RNA 
2
+ 
fluor
Oligomer 
synthesis
Robotic 
printing
Purification
PCR 
amplification

Table of contents