Experimental Surgical Models in the Laboratory Rat

Alfredo Rigalli, Veronica Di Loreto

• Publisher: CRC Press
• Year: 2016
• Print ISBN: 9781420093261
• eBook ISBN: 9781420093278
• Edition: 1
• Topic: Medicine
• Subtopic: Medical Theory, Practice & Reference

An All-Inclusive Guide to Surgical Techniques on Rats

The design of an adequate surgical model, like the choice of the animal model itself, is extremely important for obtaining reliable valuable data. Experimental Surgical Models in the Laboratory Rat summarizes a series of techniques that were applied in the Bone Biology Laboratories, School of Medicine, Rosario National University, Argentina.

Stopping just short of an exhaustive analysis of the techniques, this manual describes the basics of each surgical technique, the necessary materials, precautions to consider, the methodology to apply, and the possible results to be obtained in similar experiments.

CD-ROM: 250 Illustrations of Surgery Techniques with Step-by-Step Instruction

With succinct descriptions, color photographs, and easy-to-follow steps, the downloadable resources are a practical, full-color roadmap for performing various surgery techniques, such as catheterization, tracheostomy, intramuscular injection, subcutaneous injection, intraperitoneal injection, and oral intubation. This definitive work provides technical and practical data including volumes and concentrations of solutions, catheters used, equipment, and biological variables on the rat. Experimental Surgical Models in the Laboratory Rat is a complete reference for today's biomedical sciences investigator.

Table of contents

• Front cover
• Contents
• Preface
• Acknowledgments
• The Editors
• Contributors
• Abbreviations
• Section I: Introduction
• Chapter 1. Bioethics and Animal Care
• Chapter 2. Control of Stress and Distress
• Chapter 3. Management of Pain in Laboratory Animals
• Chapter 4. Anesthesia and Analgesia
• Chapter 5. Euthanasia
• Chapter 6. Antibiotics
• Section II: General Procedures
• Chapter 7. Operating Theatre
• Chapter 8. Rat Identification
• Chapter 9. Antisepsis, Sterilization, and Asepsis
• Chapter 10. Suture
• Chapter 11. Substances Administration
• Chapter 12. Samples
• Section III: Catheterization and Cannulation
• Chapter 13. Catheterization of the Femoral Artery and Vein
• Chapter 14. Cardiac Catheterization
• Chapter 15. Cannulation of the Thoracic Duct
• Chapter 16. Tracheostomy
• Section IV: Gastrointestinal Tract
• Chapter 17. Isolation of the Stomach
• Chapter 18. In Situ Isolation of the Intestinal Loop
• Chapter 19. Intestinal Everted Sacs
• Chapter 20. In Situ Perfusion of the Small Intestine
• Section V: Pancreas
• Chapter 21. Experimental Models for the Study of Diabetes
• Chapter 22. Islets of Langerhans Isolation
• Chapter 23. Incubation of Pancreatic Tissue Slices
• Chapter 24. In Situ Perfusion of the Pancreas
• Chapter 25. Experimental Pancreatitis
• Section VI: Liver
• Chapter 26. Hepatic Circulation Impairment
• Chapter 27. Extrahepatic Cholestasis Model
• Section VII: Ablation of Endocrine Glands
• Chapter 28. Adrenalectomy
• Chapter 29. Ovariectomy
• Chapter 30. Thyroparathyroidectomy
• Chapter 31. Parathyroid Glands
• Section VIII: Kidneys
• Chapter 32. Measurement Techniques of Renal Parameters
• Chapter 33. Kidney Isolation and Perfusion
• Chapter 34. Acute Renal Failure Models
• Chapter 35. Renal Blood Flow Measurement
• Chapter 36. Transport Studies in Plasma Membrane Vesicles Isolated from Renal Cortex
• Chapter 37. Remnant Kidney Model
• Section IX: Circulatory System
• Chapter 38. Experimental Myocardial Infarction
• Chapter 39. Experimental Arteriosclerosis
• Chapter 40. Isolated Heart: Langendorff Technique
• Section X: Additional Techniques
• Chapter 41. Polyclonal Antibodies Preparation
• Chapter 42. Histological Procedures
• Chapter 43. Bones and Bone Tissue
• Section XI: Miscellaneous
• Chapter 44. Equipment
• Chapter 45. Solution Preparation
• Chapter 46. Normal Values in IIm/Fm Rat Subline “M”
• Index
• Back cover