Gene Biotechnology
eBook - PDF

Gene Biotechnology

  1. 576 pages
  2. English
  3. PDF
  4. Available on iOS & Android
eBook - PDF

Gene Biotechnology

About this book

Covering state-of-the-art technologies and a broad range of practical applications, the Third Edition of Gene Biotechnology presents tools that researchers and students need to understand and apply today's biotechnology techniques. Many of the currently available books in molecular biology contain only protocol recipes, failing to explain the princ

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Yes, you can access Gene Biotechnology by William Wu,Helen H. Zhang,Michael J. Welsh,Peter B. Kaufman in PDF and/or ePUB format, as well as other popular books in Medicine & Biotechnology in Medicine. We have over one million books available in our catalogue for you to explore.

Information

Publisher
CRC Press
Year
2016
Print ISBN
9781032297910
eBook ISBN
9781439848326
Edition
3
Topic
Medicine
Subtopic
Biotechnology in Medicine

Table of contents

  1. Front Cover
  2. Contents
  3. Preface
  4. Authors
  5. Chapter 1: Strategies for Novel Research Projects and/or Research Grant Funding
  6. Chapter 2: Rapid Isolation of Specific cDNAs or Genes by PCR
  7. Chapter 3: Construction and Screening of Subtracted and Complete Expression cDNA Libraries
  8. Chapter 4: Subcloning of Genes or DNA Fragments
  9. Chapter 5: Nonisotopic and Isotopic DNA or RNA Sequencing
  10. Chapter 6: Bioinformation Superhighway and Computer Databases of Nucleic Acids and Proteins
  11. Chapter 7: Characterization of DNA or Genes by Southern Blot Hybridization
  12. Chapter 8: Gene Overexpression by Sense RNA in Mammalian Systems
  13. Chapter 9: Gene Underexpression in Cultured Cells and Animals by Antisense DNA and RNA Strategies
  14. Chapter 10: Analysis of Gene Expression at Functional Genomic Level Using Northern Blotting or PCR
  15. Chapter 11: Analysis of Gene Expression at Proteomic Level via Western Blotting
  16. Chapter 12: Analysis of Cellular DNA or Abundance of mRNA by Radioactivity In Situ Hybridization
  17. Chapter 13: Localization of DNA or Abundance of mRNA by Fluorescence In Situ Hybridization
  18. Chapter 14: In Situ PCR Hybridization of Low Copy Genes and in Situ RT-PCR Detection of Low Abundance mRNAs
  19. Chapter 15: Isolation and Characterization of Genes from Genomic DNA Libraries
  20. Chapter 16: Mouse Stem Cells as a Model Mammalian Cell Line for Gene Expression
  21. Chapter 17: Strategies for Gene Double Knockout
  22. Chapter 18: Large-Scale Expression and Purification of Recombinant Proteins in Cultured Cells
  23. Chapter 19: Quantitative Analysis of Functional Genome by Real-Time RT-PCR
  24. Chapter 20: High-Throughput Analysis of Gene Expression by Cutting-Edge Technology—DNA Microarrays (Gene Chips)
  25. Chapter 21: Construction and Screening of Human Antibody Libraries Using Phage Display Technology
  26. Chapter 22: Down-Regulation of Gene Expression in Mammalian Systems via siRNA Technology
  27. Chapter 23: Strategies for Gene Cloning, Expression, and Identification of Protein–Protein Interaction
  28. Chapter 24: Conditional Gene Knockout
  29. Chapter 25: How to Write a Research Manuscript for Publication in an English Journal
  30. Chapter 26: How to Protect Your Discovery and Invention : Patent 101
  31. Chapter 27: Determination of Transgene Copy Numbers and Practical Biocalculation
  32. Back Cover