PCR Technology

Current Innovations, Third Edition

Tania Nolan, Stephen A. Bustin

• Publisher: CRC Press
• Year: 2013
• eBook ISBN: 9781439848135
• Edition: 3
• Topic: Law
• Subtopic: Forensic Science
• Index: Law

PCR's simplicity as a molecular technique is, in some ways, responsible for the huge amount of innovation that surrounds it, as researchers continually think of new ways to tweak, adapt, and re-formulate concepts and applications. PCR Technology: Current Innovations, Third Edition is a collection of novel methods, insights, and points of view that

Table of contents

• Front Cover
• Contents
• Preface
• Editors
• Contributors
• Chapter 1 - Pre-PCR Processing Strategies
• Chapter 2 - Use of a Liposomal Internal Control Vehicle for Whole-Process Quality Assurance of Nucleic Acid Amplification
• Chapter 3 - Oligonucleotide Synthesis and Purification
• Chapter 4 - Augmenting PCR: Effects of Additives in the Polymerase Chain Reaction
• Chapter 5 - Real-Time Fluorescent PCR by Labeled Primer with a Single Fluorescent Molecule
• Chapter 6 - Using Scorpion Primers for Genotyping
• Chapter 7 - LNA™: Adding New Functionality to PCR
• Chapter 8 - Modified dNTPs: A Toolbox for Use in PCR
• Chapter 9 - Thermocycler Calibration and Analytical Assay Validation
• Chapter 10 - Ultra-High-Speed PCR Instrument Development
• Chapter 11 - Dropletization of Bioreactions: How Single-Cell Quantitative Analyses May Improve Gene Measurement and Cancer Diagnosis
• Chapter 12 - Assay Design for Real-Time qPCR
• Chapter 13 - Primer Design for Large-Scale Multiplex PCR and Arrayed Primer Extension
• Chapter 14 - Development and Use of qPCR Assays for Detection and Study of Neglected Tropical and Emerging Infectious Diseases
• Chapter 15 - MIQE: Guidelines for Reliable Design and Transparent Reporting of Real-Time PCR Assays
• Chapter 16 - Hypothesis-Driven Approaches to Multivariate Analysis of qPCR Data
• Chapter 17 - Experiment Design, Data Management, and Univariate Statistical Analysis of Gene-Expression Data Obtained by Real-Time Quantitative PCR
• Chapter 18 - Biomarker Discovery via RT-qPCR and Bioinformatical Validation
• Chapter 19 - Optimization of Quantitative Real-Time PCR for Studies in Cartilage Mechanobiology
• Chapter 20 - Enriching DNA Sequences with Nucleotide Variation by Thymidine Glycosylases Combined with Suppression PCR
• Chapter 21 - Chromosome Walking by Inverse PCR
• Chapter 22 - Expression Profiling of MicroRNAs by Quantitative Real-Time PCR: The Good, the Bad, and the Ugly
• Chapter 23 - Dye-Based High-Throughput qPCR in Microfluidic Platform BioMark™
• Chapter 24 - Surface Plasmon Resonance-Based Biosensor Technology for Real-Time Detection of PCR Products
• Chapter 25 - Current Innovations in the Development and Application of qPCR in a Resource-Limited Setting
• Chapter 26 - A Review of Isothermal Nucleic Acid Amplification Technologies
• Chapter 27 - Proximity Ligation Assay for Protein Quantification
• Chapter 28 - High-Resolution Melt Analysis
• Back Cover