Real-time PCR
eBook - ePub

Real-time PCR

  1. 333 pages
  2. English
  3. ePUB (mobile friendly)
  4. Available on iOS & Android
eBook - ePub

Real-time PCR

About this book

With a variety of detection chemistries, an increasing number of platforms, multiple choices for analytical methods and the jargon emerging along with these developments, real-time PCR is facing the risk of becoming an intimidating method, especially for beginners. Real-time PCR provides the basics, explains how they are exploited to run a real-time PCR assay, how the assays are run and where these assays are informative in real life. It addresses the most practical aspects of the techniques with the emphasis on 'how to do it in the laboratory'. Keeping with the spirit of the Advanced Methods Series, most chapters provide an experimental protocol as an example of a specific assay.

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Yes, you can access Real-time PCR by M Dorak, M Tevfik Dorak,M Dorak, M Tevfik Dorak in PDF and/or ePUB format, as well as other popular books in Medicine & Biotechnology in Medicine. We have over one million books available in our catalogue for you to explore.

Information

Publisher
Taylor & Francis
Year
2007
eBook ISBN
9781134183999
Edition
1
Topic
Medicine
Subtopic
Biotechnology in Medicine

Table of contents

  1. Cover Page
  2. Title Page
  3. Copyright Page
  4. Contributors
  5. Abbreviations
  6. Preface
  7. Glossary
  8. Links to Software and Web Resources Cited
  9. 1 An Introduction to Real-time PCR
  10. Protocol 1.1: Treatment of Total RNA with RNase-free DNase I
  11. Protocol 1.2: Hybrid Protocol for the Isolation of Total RNA
  12. Protocol 1.3: Two-step Master Mix
  13. Protocol 1.4: Two-step PCR Master Mix for Probe-based Assays
  14. Protocol 1.5: Two-step PCR Master Mix – Primer or SYBR® Green I Based Assay
  15. 2 Data Analysis and Reporting
  16. 3 Relative Quantification
  17. 4 Normalization
  18. 5 High-throughput Primer and Probe Design
  19. 6 Quantitative Analysis of Ocular Gene Expression
  20. 7 Quantitative Gene Expression by Real-time PCR: A Complete Protocol
  21. 8 Real-time PCR Using SYBR® Green
  22. 9 High-resolution Melting Analysis for Scanning and Genotyping
  23. 10 Quantitative Analyses of DNA methylation
  24. Protocol 10.1
  25. 11 Mitochondrial DNA Analysis
  26. 12 Real-time Immuno-PCR
  27. Protocol 12.1: Basic Real-time Immuno-PCR Protocol
  28. 13 Clinical Microbiology
  29. Protocol 13.1: Detection of Prevotella Intermedia by Real-time PCR Assay
  30. 14 Clinical Virology
  31. Protocol 14.1: Detection of Influenza A by a 5’ Nuclease Real-time RT-PCR Assay
  32. Protocol 14.2: Detection and Differentiation of Herpes Simplex Virus Types 1 and 2 Using a Hybridization Probe Real-time PCR assay
  33. Protocol 14.3: Quantitative Analysis of BKV Load by a 5’ Nuclease Real-time PCR (BKV-Tag-qPCR) Assay
  34. Protocol 14.4: Real-time PCR Internal Control (Niesters, 2004)
  35. Troubleshooting Guide 14.1: False-negative Result in the WhSl-para3–5N Assay
  36. Troubleshooting Guide 14.2: Variation in Fluorescent Signal from Positive Specimens in the RSV MGB Assay
  37. Troubleshooting Guide 14.3: Uncharacteristic Herpes Simplex Virus Type 2 Melting Curves
  38. Troubleshooting Guide 14.4: Underestimation of BK Viral Load due to Sequence Variation
  39. 15 Solid Organ Transplant Monitoring
  40. Protocol 15.1: Monitoring Solid Organ Transplant Recipients for Rejection
  41. 16 Real-time PCR Applications in Hematology
  42. Protocol 16.1: Post-transplant MRD Monitoring
  43. Protocol 16.2: Use of TSPY Repeats on Y-chromosome in Chimerism Studies
  44. 17 Real-time PCR for Prenatal Diagnosis of Monogenic Diseases Caused by Single Nucleotide Changes – The Example of the Hemoglobinopathies
  45. Protocol 17.1: Cleaning Chorionic Villi (CVS) by Microscopic Dissection
  46. Protocol 17.2: Centrifugation of Amniotic Fluid Cell Samples to Collect Amniocytes
  47. Protocol 17.3: DNA Extraction from Chorionic Villi Samples or Amniocytes Using Commercially Available DNA Purification Columns
  48. Protocol 17.4: Protocol for Rapidly Screening Multiple β-globin Gene Mutations by Real-time PCR: Application to Carrier Screening and Prenatal Diagnosis of Thalassemia Syndromes
  49. Protocol 17.5: Real-time PCR Protocols for Singlecell Genotyping and PGD
  50. Protocol 17.6: Nested PCR and genotyping of β-globin gene using Real-Time PCR in the LightCycler®
  51. Protocol 17.7: Monitoring of Contamination by Sizing GABRB3 and D13S314 Polymorphic Microsatellites
  52. Protocol 17.8: Technical Tips and Troubleshooting when Performing Real-time PCR and Genotyping Using the LightCycler® System 1.0 and 1.5