Functional Protein Microarrays in Drug Discovery

Paul F. Predki

• Publisher: CRC Press
• Year: 2007
• Print ISBN:
  9780849398094
• eBook ISBN:
  9781420009842
• Edition: 1
• Topic: Medicine
• Subtopic: Biochemistry in Medicine

An ideal text for biotechnologists, protein chemists, and biochemists, Functional Protein Microarrays in Drug Discovery explores all aspects of functional protein microarrays, including basic principles, methods, and applications. The book discusses the generation of functional protein content and describes both standard and state-of-the-art fabrication methods. It reviews current and next generation approaches to assay detection and presents a wide range of applications, from biomolecular interaction discovery and characterization to immune response profiling. The book also addresses several fundamental computational issues, bioinformatics and data analysis.

Table of contents

• Front cover
• Table of Contents
• Preface
• Contributors List
• Introduction
• Section 1: Functional Protein Content for Microarrays
• Chapter 1. High-Throughput Gene Cloning Using the Gateway® Technology
• Chapter 2. Protein Expression for MicroArrays
• Chapter 3. EmergingTrend:Cell-Free Protein Expression
• Section 2: Fabrication of Functional Protein Microarrays
• Chapter 4. The Critical Role of Surface Chemistry in Protein Microarrays
• Chapter 5. Fabrication of Sol-Gel- Derived Protein Microarrays for Diagnostics and Screening
• Chapter 6. Printing and QC of Functional Protein Microarrays
• Chapter 7. Protein Engineering for Surface Attachment
• Chapter 8. Protein In Situ Arrays through Cell-Free Protein Synthesis
• Section 3: Detection Methods for Protein Microarrays
• Chapter 9. Fluorescent Detection Methods for Protein Microarrays
• Chapter 10. Functional Analysis of Protein Interactions Using Surface Plasmon Resonance-Based Microarrays
• Chapter 11. Leaving the Surface Behind: At the Intersection of Protein Microarrays and Mass Spectrometry
• Chapter 12. High-Resolution Label- Free Detection Applied to Protein Microarray Research
• Section 4: Applications of Functional Protein Microarrays
• Chapter 13. Studying Protein–Protein Interactions with Protein Microarrays: Rapid Identification of 14-3-3 Protein Binding Partners
• Chapter 14. A Combined Force of Chemical Genetics and Protein Microarrays
• Chapter 15. Antibody Profiling for Protein Drug Development and Clinical Development
• Chapter 16. Humoral Response Profiling Using Protein Microarrays
• Chapter 17. DNA Interactions with Arrayed Proteins
• Chapter 18. G Protein–Coupled Receptor Microarrays for Drug Discovery
• Chapter 19. Kinase Substrate Identification Using Yeast Protein Microarrays
• Section 5: Bioinformatics & Data Analysis
• Chapter 20. Protein Microarray Image Analysis
• Chapter 21. The Analysis of Protein Arrays
• Chapter 22. Evaluating Precision and Recall in Functional Protein Arrays
• Chapter 23. Visualization of Protein Microarray Data
• Index
• Back cover