Cell-Free Protein Expression

W. Antoni Kudlicki

• Publisher: CRC Press
• Year: 2007
• eBook ISBN: 9781498713290
• Edition: 1
• Topic: Biological Sciences
• Subtopic: Biology
• Index: Biological Sciences

Following its inception in the 1950s, cell-free protein synthesis made a tremendous impact on the basic life sciences. The use of cell-free systems was key to understanding molecular mechanisms underlying one of the most complicated processes found in nature: protein translation. Since this time, aggressive cutting-edge research and stiff commerica

Table of contents

• Cover
• Title Page
• Copyright Page
• Contents
• Preface
• 1. The Role of Cell-Free Rabbit Reticulocyte Expression Systems in Functional Proteomics
• 2. Advances in Insect-Based Cell-Free Protein Expression
• 3. Advantages and Applications of the Batch-Formatted E.coli Cell-Free Expression System
• 4. Energetics in Escherichia coli-Based Batch Cell-Free Systems
• 5. Disulfide Bond Formation in Bacteria-Based Cell-Free Protein Expression
• 6. The PURE System: A Minimal Cell-Free Translation System
• 7. Cell-Free Expression Approaches for the Production and Characterization of Membrane Proteins
• 8. Cell-Free Expression for Protein NMR
• 9. Cell-Free Synthesis of Membrane Proteins for X-Ray Crystallography
• 10. Bacterial Cell-Free Expression Systems for High-Throughput Protein Production
• 11. Cell-Free Protein Synthesis for Protein Microarrays
• 12. Cell-Free Protein Expression Screening and Protein Immobilization Using Protein Microarrays
• 13. Cell-Free Protein Expression Labeling with Fluorophores
• 14. Cell-Free Synthesis of Defined Protein Conjugates by Site-Directed Cotranslational Labeling
• 15. C-Terminal Labeling of Proteins Using Fluorescently Conjugated Puromycin Derivatives
• 16. Translation Engineering and Synthetic Biology
• 17. Accelerated Protein Evolution Using Ribosome Display
• 18. Application of in Vitro Virus ( IVV ) Technique for High-Throughput Analysis of Protein-Protein Interactions
• Index