Genome Engineering via CRISPR-Cas9 System

Vijai Singh, Pawan K. Dhar

• Publisher: Academic Press
• Year: 2020
• Print ISBN: 9780128181409
• eBook ISBN: 9780128181416
• Topic: Biological Sciences
• Subtopic: Biotechnology
• Index: Biological Sciences

Genome Engineering via CRISPR-Cas9 Systems presents a compilation of chapters from eminent scientists from across the globe who have established expertise in working with CRISPR-Cas9 systems. Currently, targeted genome engineering is a key technology for basic science, biomedical and industrial applications due to the relative simplicity to which they can be designed, used and applied. However, it is not easy to find relevant information gathered in a single source. The book contains a wide range of applications of CRISPR in research of bacteria, virus, algae, plant and mammalian and also discusses the modeling of drosophila, zebra fish and protozoan, among others. Other topics covered include diagnosis, sensor and therapeutic applications, as well as ethical and regulatory issues. This book is a valuable source not only for beginners in genome engineering, but also researchers, clinicians, stakeholders, policy makers, and practitioners interested in the potential of CRISPR-Cas9 in several fields. - Provides basic understanding and a clear picture on how to design, use and implement the CRISPR-Cas9 system in different organisms - Explains how to create an animal model for disease research and screening purposes using CRISPR - Discusses the application of CRISPR-Cas9 systems in basic sciences, biomedicine, virology, bacteriology, molecular biology, neurology, cancer, industry, and many more

Table of contents

• Cover image
• Title page
• Table of Contents
• Copyright
• Dedication
• Contributors
• About the editors
• Foreword
• Preface
• Acknowledgments
• Chapter 1. An introduction to genome editing CRISPR-Cas systems
• Chapter 2. Evolution and molecular mechanism of CRISPR/Cas9 systems
• Chapter 3. Exploring the potential of CRISPR-Cas9 for the removal of human viruses
• Chapter 4. Programmable removal of bacterial pathogens using CRISPR-Cas9 system
• Chapter 5. Targeted genome editing using CRISPR/Cas9 system in fungi
• Chapter 6. CRISPR-Cas9 system for fungi genome engineering toward industrial applications
• Chapter 7. Development and challenges of using CRISPR-Cas9 system in mammalians
• Chapter 8. CRISPR-Cas9 system “a mighty player in cancer therapy”
• Chapter 9. CRISPR-Cas9 for therapy: the challenges and ways to overcome them
• Chapter 10. Engineering of Cas9 for improved functionality
• Chapter 11. The current progress of CRISPR/Cas9 development in plants
• Chapter 12. Fruit crops improvement using CRISPR/Cas9 system
• Chapter 13. CRISPR/Cas9 engineered viral immunity in plants
• Chapter 14. Genome engineering in medicinally important plants using CRISPR/Cas9 tool
• Chapter 15. Genome editing of algal species by CRISPR Cas9 for biofuels
• Chapter 16. Development and use of CRISPR in industrial applications
• Chapter 17. Functional understanding of CRISPR interference: its advantages and limitations for gene silencing in bacteria
• Chapter 18. Genome engineering in insects: focus on the CRISPR/Cas9 system
• Chapter 19. Recent progress of CRISPR-Cas9 in zebra fish
• Chapter 20. CRISPR: a revolutionary tool for genome engineering in the protozoan parasites
• Chapter 21. Emergent challenges for CRISPR: biosafety, biosecurity, patenting, and regulatory issues
• Appendices
• Glossary
• Author Index
• Subject Index