Methods in Neurosciences
eBook - ePub

Methods in Neurosciences

Electrophysiology and Microinjection

  1. 520 pages
  2. English
  3. ePUB (mobile friendly)
  4. Available on iOS & Android
eBook - ePub

Methods in Neurosciences

Electrophysiology and Microinjection

About this book

Methods in Neurosciences, Volume 4: Electrophysiology and Microinjection is a collection of papers that deals with the electrically excitability feature of many cell types. This volume describes the characteristic features of some nervous tissue to conduct signals along cellular paths or ""wires."" The text presents such paths as a way stimuli are transferred in the nervous system. Section I reviews the recording methodologies such as those used in measuring noninactivating calcium current in smooth muscles cells or the two-suction electrode voltage-clamp recording. Section II deals in detail with voltage clamping and voltammetry; the text also explains the practical steps in using the current pump-assisted voltage clamp. One paper examines the X-ray microprobe analysis of voltage clamped single heart ventricular myocytes, while another paper explains in vivo voltammetry. Section III addresses electrophysiology and purification of specific receptors; one paper presents the results of an electrophysiological study of hormone secretion by single adenohypophyseal cells. Section IV describes special electrodes and equipment, while Section V deals with special preparations needed in culture preparation or in the study of pharmacology of excitatory amino acids on neurons found in the central nervous system. Chapter VI addresses data analysis and reduction such as digital filtering of bioelectric potentials in personal computers. This book will prove valuable for microbiologists, cellular scientists, microchemists, and academicians working in the fields of neuroscience.

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Yes, you can access Methods in Neurosciences by P. Michael Conn in PDF and/or ePUB format, as well as other popular books in Biological Sciences & Zoology. We have over one million books available in our catalogue for you to explore.

Information

Publisher
Academic Press
Year
2013
Print ISBN
9780121852573
eBook ISBN
9781483269368
Topic
Biological Sciences
Subtopic
Zoology
Index
Biological Sciences
Index

A

A current channels, unitary recordings, 313–317
Adenohypophyseal cells, membrane capacitance
dual-phase lock-in amplifiers for, 197–201
macroscopic changes
compensated, 203–206
noncompensated, 207–208
microscopic measurements, 201–203
minimal electrical circuit, 195–196
phase-shift errors, 201
small change measurement, 197–201
time-domain technique, 195–197
Affinity chromatography, CCDR on WGA–Sepharose 4B, 215–216
Aliasing, 398
Alkaike information criterion, 424–425
Amplifiers, two-phase lock-in, 197–201
Amplitude measurements, in LPROC, 482
Analog filters, 398
Analog-to-digital conversion, 397–398
Artifacts, stimulus, digital filters for, 407–408
Artificial cerebrospinal fluid, 303, 313, 357–358
Ascorbic acid, signal identification, 129–130
Asymptotic information criterion, 434
Axons, steady-state current and isotope flux, pump-clamp technique for, 90–92

B

Bath application method, for ICa(ni), 48–52
Bay K 8644, effects on noninactivating Ca2+ current (smooth muscle), 49–50
Biomembranes, sarcoplasmic reticulum vesicles incorporation into giant proteoliposomes, 376–377
preparation, 375
BMDP statistical package, 413, 423
Burst analysi...

Table of contents

  1. Cover image
  2. Title page
  3. Table of Contents
  4. Copyright page
  5. Contributors to Volume 4
  6. Preface
  7. Methods in Neurosciences
  8. Section I: Recording Methodologies
  9. Section II: Voltage Clamping and Voltammetry
  10. Section III: Electrophysiology and Purification of Specific Receptors
  11. Section IV: Special Electrodes and Equipment
  12. Section V: Special Preparations
  13. Section VI: Data Analysis and Reduction
  14. Index