Cell Culture
eBook - ePub

Cell Culture

  1. 440 pages
  2. English
  3. ePUB (mobile friendly)
  4. Available on iOS & Android
eBook - ePub

Cell Culture

About this book

Methods in Neurosciences, Volume 3: Quantitative and Qualitative Microscopy is a collection of papers that deals with microscopic techniques in statistical measures. This volume describes microscopy using sophisticated stains and dyes to advance observation of tests and experiments. Section I describes autoradiography including micro chemical methods, high-resolution autoradiography, and single- or double-label quantitative autoradiography for use in imaging of brain activity patterns or determining cerebral physiology. Section II discusses the quantification of structures through statistical and computational methods including dynamic video imaging technology. Section III explains the use of tracers, toxins, or dyes in tracing neuronal connections. One paper addresses the use of small injections of axonally transported fluorescent tracers. Section IV explains staining technology such as using the silver impregnation method for frozen sections of human nervous tissue that are gathered from tissues preserved in formalin. Section V addresses freezing techniques and those using freeze-fracture methods in neurobiology. The text also discusses cryoprotection and other freezing methods to control ice crystals found in fixed or unfixed brain tissues. Section VI presents the combined and high-resolution methods in polarization microscopy and microscopic investigations. Cellular biologists, micro-chemists, and scientific researchers in the field of micro- and cellular biology will appreciate this book.

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Yes, you can access Cell Culture by P. Michael Conn in PDF and/or ePUB format, as well as other popular books in Biological Sciences & Science Research & Methodology. We have over one million books available in our catalogue for you to explore.
Index

A

Acetylcholine
metabolism by primary cultured neurons during in vitro growth, 105–208
receptor levels in microcarrier-based cultures of nerve cells, myoblasts, and autologous nerve–muscle cells, 206
Adenohypophyseal tissue
in vivo culture
applications, 322–328
infusion/withdrawal system, 317–320
maintenance of catheter patency, 321
number of, 321
transplantation into hypophysectomized hosts
procedure, 317
surgical precautions, 321
survival of transplant, 320–321
verification of hypophysectomy, 322
Affinity methods, purification of neurons from chick sympathetic ganglia, 155
Agar medium, semisolid, culture of glioma cells in, 227
Aggregates
fetal rat brain cells
in stationary culture, 219–220
as targets in confrontation cultures, 225–226
formation in neuronal and muscle microcarrier cultures, 202–203
D-Ala2-D-Leu5-enkephalin, chronic exposure of cord-DRG expiants to, 80
Amino acid sequences
bombesin, 342
epidermal growth factor (human), 342
gastrin-releasing peptide (human), 342
γ-Aminobutyric acid
differential sensitivity of sensory-evoked synaptic network responses of cord-DRG explants, 78
effect on [3H]flunitrazepam binding to primary cultured neurons, 108 metabolism by primary cultured neurons during
in vitro growth, 105–208
Amino-dextran carriers...

Table of contents

  1. Cover image
  2. Title page
  3. Table of Contents
  4. Copyright page
  5. Contributors to Volume 2
  6. Preface
  7. Methods in Neurosciences
  8. Section I: General Approaches to Preparation of Cell Cultures
  9. Section II: Purification of Cell Types
  10. Section III: Bulk Isolation
  11. Section IV: Special Approaches
  12. Section V: Functional Assessment
  13. Section VI: Cell Lineage
  14. Index