Gene Transfer and Expression in Mammalian Cells
eBook - ePub

Gene Transfer and Expression in Mammalian Cells

  1. 722 pages
  2. English
  3. ePUB (mobile friendly)
  4. Available on iOS & Android
eBook - ePub

Gene Transfer and Expression in Mammalian Cells

About this book

The production of proteins in mammalian cells is an important tool in numerous scientific and commercial areas. For example, proteins for human therapy, vaccination or diagnostic applications are typically produced in mammalian cells. Gene cloning, protein engineering, biochemical and biophysical characterization of proteins also require the use of gene expression in mammalian cells. Other applications in widespread use involve screening of libraries of chemical compounds in drug discovery, and the development of cell-based biosensors.This book presents a state-of-the-art comprehensive coverage of the technical aspects of gene expression in mammalian cells, written by experienced scientists working at the forefront of the field.

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Yes, you can access Gene Transfer and Expression in Mammalian Cells by S.C. Makrides in PDF and/or ePUB format, as well as other popular books in Biological Sciences & Genetics & Genomics. We have over one million books available in our catalogue for you to explore.
Subject Index
Accessory genes 29
N-Acetylglucosamine (GlcNAc) 434, 435
O-Ξ²-N-Acetylglucosaminyltransferases (OGT) 444
Acquired immunodeficiency syndrome (AIDS) 88, 169
criteria for mucosal HIV vaccine 172–173
intrabodies in anti-HIV research 580–581
live-attenuated Sabin poliovirus as AIDS vaccine vector 174
mucosal immune system and vaccine development 173
sexual transmission 171–172
use of poliovirus vectors in developing countries 174
Adeno-associated virus (AAV)
as gene delivery vector 11
biology 93–97
electron microscope image 94
life cycles 96
development of new vectors 103–105
immunological aspects 102–103
in vitro and in vivo gene transfer 99–102
production of recombinant vectors 97–99
Adenoviridae family 10
Adenovirus (Ad) 109–111, 122
as gene delivery vector 10
gene delivery
capsid proteins 115
cell entry pathway 115–116
cell receptors 115–116
conjugate targeting system 118
genetic targeting system 118–119
infection mechanisms 116
targeting 116–119
gene expression
high-level expression 119–120
specific transgene expression 120–121
tissue-specific promoters 120
tumor-specific promoters 120–121
genome 110
production and analysis of virus particles 121–122
vectors 110, 111
first generation 111–112
replication 114
second generation 112–113
third generation 113–114
Adult T-cell leukemia (ATL) 581
Aequorin 294
Affinity chromatography 649–650
fusion proteins 650–651
high-resolution chromatography 651–652
African green monkey CV1 kidney cell line (COS) 2, 9, 84
COS-7 cells 76, 77, 79, 84, 85, 215
African green monkey kidney cell line (Vero) 2
Alkaline phosphatase (AP) 303–304
Alpha fetoprotein (AFP) promoter 120
Alphaviruses 189–190
effects of alphaviruses and replicons on infected cells 193–194
future perpectives 202–203
Alzheimer's disease 582
Aminoglycoside phosphotransferase (neo) gene 274
(Β±)-N-(3-Aminopropyl-N,N-dimethyl-2,3-bis(dodecyloxy)-1-propanammonium bromide (GAP-DLRIE) 280, 281
Amphotropic envelope of MLV (Ampho) 259–260
Amplicons 35, 36
Amyloid precursor protein (APP) 216
Antibiotic-responsive systems 592
erythromycin-response gene expression (E.REX) 594–595
streptogramin-responsive gene expression (PIP systems) 593–594
tetracycline-responsive gene expression 592–593
...

Table of contents

  1. Cover image
  2. Title page
  3. Table of Contents
  4. Preface
  5. Why choose mammalian cells for protein production?
  6. Vectors for gene expression in mammalian cells
  7. Virus-based vectors for gene expression in mammalian cells: Herpes simplex virus
  8. Virus-based vectors for gene expression in mammalian cells: Epstein-Barr virus
  9. Virus-based vectors for gene expression in mammalian cells: SV40
  10. Virus-based vectors for gene expression in mammalian cells: Adeno-associated virus
  11. Virus-based vectors for gene expression in mammalian cells: Adenovirus
  12. Virus-based vectors for gene expression in mammalian cells: Vaccinia virus
  13. Virus-based vectors for gene expression in mammalian cells: Baculovirus
  14. Virus-based vectors for gene expression in mammalian cells: Coronavirus
  15. Virus-based vectors for gene expression in mammalian cells: Poliovirus
  16. Virus-based vectors for gene expression in mammalian cells: Sindbis virus
  17. Virus-based vectors for gene expression in mammalian cells: Semliki Forest virus
  18. Virus-based vectors for gene expression in mammalian cells: Retrovirus
  19. Virus-based vectors for gene expression in mammalian cells: Lentiviruses
  20. Methods for DNA introduction into mammalian cells
  21. Lipid reagents for DNA transfer into mammalian cells
  22. Reporter genes for monitoring gene expression in mammalian cells
  23. Gene transfer and gene amplification in mammalian cells
  24. Co-transfer of multiple plasmids/viruses as an attractive method to introduce several genes in mammalian cells
  25. Optimization of plasmid backbone for gene expression in mammalian cells
  26. Use of scaffold/matrix-attachment regions for protein production
  27. Chromatin insulators and position effects
  28. Locus control regions
  29. Protein synthesis, folding, modification, and secretion in mammalian cells
  30. Pathways and functions of mammalian protein glycosylation
  31. Metabolic engineering of mammalian cells for higher protein yield
  32. Translational regulation in mammalian cells
  33. Pathways of mammalian messenger RNA degradation
  34. Pathways of mammalian protein degradation
  35. Stabilization of proteasomal substrates by viral repeats
  36. Architecture and utilization of highly expressed genomic sites
  37. Intracellular targeting of antibodies in mammalian cells
  38. Inducible gene expression in mammalian cells
  39. Protein production by large-scale mammalian cell culture
  40. Protein production in transgenic animals
  41. Strategies for the purification of recombinant proteins
  42. Subject Index