Quantitative and Qualitative Microscopy

P. Michael Conn

• Publisher: Academic Press
• Year: 2013
• Print ISBN: 9780121852559
• eBook ISBN: 9781483268170
• Topic: Biological Sciences
• Subtopic: Biology
• Index: Biological Sciences

Methods in Neurosciences, Volume 3: Quantitative and Qualitative Microscopy is a collection of papers that deals with microscopic techniques in statistical measures. This volume describes microscopy using sophisticated stains and dyes to advance observation of tests and experiments. Section I describes autoradiography including micro chemical methods, high-resolution autoradiography, and single- or double-label quantitative autoradiography for use in imaging of brain activity patterns or determining cerebral physiology. Section II discusses the quantification of structures through statistical and computational methods including dynamic video imaging technology. Section III explains the use of tracers, toxins, or dyes in tracing neuronal connections. One paper addresses the use of small injections of axonally transported fluorescent tracers. Section IV explains staining technology such as using the silver impregnation method for frozen sections of human nervous tissue that are gathered from tissues preserved in formalin. Section V addresses freezing techniques and those using freeze-fracture methods in neurobiology. The text also discusses cryoprotection and other freezing methods to control ice crystals found in fixed or unfixed brain tissues. Section VI presents the combined and high-resolution methods in polarization microscopy and microscopic investigations. Cellular biologists, micro-chemists, and scientific researchers in the field of micro- and cellular biology will appreciate this book.

Addendum

Addendum to Article [6]

W.T. Mason, J. Hoyland, S.R. Rawlings and G.T. Relf

Note added in proof. Since this manuscript was prepared, an excellent two-volume monograph has been published which expands on a number of points made here [Y. L. Wang and O. L. Taylor, eds., Methods Cell Biol. 29, 1989; O. L. Taylor and Y. L. Wang, eds., Methods Cell Biol. 30, 1989].

Acknowledgments

We would like to acknowledge the generous financial support of the Agricultural and Food Research Council, the Nutfield Foundation, Wellcome Trust, Kabi Vitrum AB of Stockholm, and Joyce-Loebl Ltd. of Gateshead, U.K. We also would particularly thank Dr. Craig B. Neylon and Dr. R. F. Irvine for allowing us to use some of their unpublished material.

Index

A

Abrin, mode of action, 262

4-Acetamido-4′-isothiocyanostilbene-2,2′-disulfonic acid

advantages and disadvantages, 321

combination with other techniques, 319

as fluorescent whole cell stain, 319–321

in situ for Golgi-like stain, 317–319

retrograde axonal transport, 319–321

for secondary antibody labeling, 319

structural formula for, 318

uses of, 328–329

Ammoniacal silver–dichromate solution

preparation, 331

silver impregnation of frozen sec...

Table of contents

• Cover image
• Title page
• Table of Contents
• Copyright page
• Contributors to Volume 3
• Preface
• Methods in Neurosciences
• Section I: Autoradiography
• Section II: Quantification of Structures: Statistical and Computational Methods
• Section III: Tracing Neuronal Connections: Tracers, Toxins, and Dyes
• Section IV: Staining Technology
• Section V: Freezing Techniques
• Section VI: Combined and High-Resolution Methods
• Addendum