RNA Methodologies

Laboratory Guide for Isolation and Characterization

Robert E. Farrell Jr., Robert E. Farrell, Jr.

• Publisher: Academic Press
• Year: 2009
• Print ISBN:
  9780123747273
• Edition: 4
• eBook ISBN:
  9780080884950
• Topic: Biological Sciences
• Subtopic: Cell Biology
• Index: Biological Sciences

This is the fourth edition of the successful laboratory guide which has translated the rich story of riboneucleic acid for over fifteen years. RNA Methodologies 4e presents the latest collection of tested laboratory protocols for the isolation and characterization of eukaryotic and prokaryotic RNA with greater emphasis on transcript profiling, including quantification issues and elucidation of alternative transcription start sites. Collectively the chapters work together providing analysis with clear take-home lessons to assist researchers to understand RNA and to optimize time at the bench. The abundant use of flow charts, tables and graphs are especially helpful in the planning and implementation phases of a project and facilitate learning. 30% new material in this edition includes the addition of RNA isolation protocols including RNA isolation from tissue, expansion of PCR optimization analysis and RNA interference sections, the introduction of a new chapter dealing with the molecular biology of plants, and an expanded glossary. - 30% new material with the addition of RNA isolation protocols including RNA isolation from tissue, expansion of PCR optimization analysis and RNA interference sections, the introduction of a new chapter dealing with the molecular biology of plants, and an expanded glossary - Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center - Includes classic and contemporary techniques useful for all labs

Table of contents

• Cover image
• Table of Contents
• Copyright
• Dedication
• Preface
• Chapter 1. RNA and the Cellular Biochemistry Revisited
• Chapter 2. RNA Isolation Strategies
• Chapter 3. The Truth about Tissues
• Chapter 4. Going Green
• Chapter 5. Isolation of Polyadenylated RNA
• Chapter 6. Quality Control for RNA Preparations
• Chapter 7. Resilient Ribonucleases
• Chapter 8. Stringency
• Chapter 9. Electrophoresis of RNA
• Chapter 10. Photodocumentation and Image Analysis
• Chapter 11. Northern Analysis
• Chapter 12. Nucleic Acid Probe Technology
• Chapter 13. Practical Nucleic Acid Hybridization
• Chapter 14. Principles of Detection
• Chapter 15. Quantification of Specific mRNAs by Nuclease Protection
• Chapter 16. Analysis of Nuclear RNA
• Chapter 17. cDNA Synthesis
• Chapter 18. RT-PCR
• Chapter 19. Quantitative PCR Techniques
• Chapter 20. Functional Genomics and Transcript Profiling
• Chapter 21. High-Throughput Analysis of Gene Expression
• Chapter 22. Non-Array Methods for Global Analysis of Gene Expression
• Chapter 23. RNAi
• Chapter 24. Genomes, Transcriptomes, Proteomes, and Bioinformatics
• Chapter 25. An RNA Paradigm
• Epilogue A Few Pearls of Wisdom
• Appendix A. Maintaining Complete and Accurate Records
• Appendix B. Converting Mass to Moles
• Appendix C. Useful Stock Solutions for the Molecular Biologist
• Appendix D. Phenol Preparation
• Appendix E. Disposal of Ethidium Bromide and SYBR Green Solutions
• Appendix F. DNase I Removal of DNA from an RNA Sample
• Appendix G. RNase Incubation to Remove RNA from a DNA Sample
• Appendix H. Deionization of Formamide, Formaldehyde, and Glyoxal
• Appendix I. Silanizing Centrifuge Tubes and Glassware
• Appendix J. Trypsinization Protocol for Anchorage-Dependent Cells
• Appendix K. Isolation of High-Molecular-Weight DNA by Salting-Out
• Appendix L. Electrophoresis: Principles, Parameters, and Safety
• Appendix M. Polyacrylamide Gel Electrophoresis
• Appendix N. Dot Blot Analysis
• Appendix O. Centrifugation as a Mainstream Tool for the Molecular Biologist
• Appendix P:. Selected Suppliers of Equipment, Reagents, and Services
• Appendix Q. Useful SI Units
• Appendix R. Common Abbreviations
• Appendix S. Trademark Citations
• Glossary
• Index