Experimental Manipulation of Gene Expression

Masayori Inouye

• Publisher: Academic Press
• Year: 2014
• Print ISBN:
  9780123723802
• eBook ISBN:
  9781483273976
• Topic: Biological Sciences
• Subtopic: Biophysics
• Index: Biological Sciences

Experimental Manipulation of Gene Expression discusses a wide range of host systems in which to clone and express a gene of interest. The aims are for readers to quickly learn the versatility of the systems and obtain an overview of the technology involved in the manipulation of gene expression. Furthermore, it is hoped that the reader will learn enough from the various approaches to be able to develop systems and to arrange for a gene of particular interest to express in a particular system. The book opens with a chapter on the design and construction of a plasmid vector system used to achieve high-level expression of a particular phage regulatory protein normally found in minute amounts in a phage-infected bacterial cell. This is followed by separate chapters on topics such as high-level expression vectors that utilize efficient Escherichia coli lipoprotein promoter as well as various other portions of the lipoprotein gene Ipp; DNA cloning systems for streptomycetes; and the design and application of vectors for high-level, inducible synthesis of the product of a cloned gene in yeast.

Table of contents

• Cover image
• Title page
• Table of Contents
• Copyright
• Contributors
• Preface
• Chapter 1: Use of Phage λ Regulatory Signals to Obtain Efficient Expression of Genes in Escherichia coli
• Chapter 2: Multipurpose Expression Cloning Vehicles in Escherichia coli
• Chapter 3: Molecular Cloning in Bacillus subtilis
• Chapter 4: Developments in Streptomyces Cloning
• Chapter 5: Vectors for High-Level, Inducible Expression of Cloned Genes in Yeast
• Chapter 6: Genetic Engineering of Plants by Novel Approaches
• Chapter 7: λSV2, a Plasmid Cloning Vector that Can Be Stably Integrated in Escherichia coli
• Chapter 8: Construction of Highly Transmissible Mammalian Cloning Vehicles Derived from Murine Retroviruses
• Chapter 9: Use of Retrovirus-Derived Vectors to Introduce and Express Genes in Mammalian Cells
• Chapter 10: Production of Posttranslationally Modified Proteins in the SV40–Monkey Cell System
• Chapter 11: Adenovirus Type 5 Region-E1A Transcriptional Control Sequences
• Chapter 12: Expression of Proteins on the Cell Surface Using Mammalian Vectors
• Chapter 13: Expression of Human Interferon-γ in Heterologous Systems
• Chapter 14: Commercial Production of Recombinant DNA-Derived Products
• Appendix 1: Two-Dimensional DNA Electrophoretic Methods Utilizing in Situ Enzymatic Digestions
• Appendix 2: Site-Specific Mutagenesis Using Synthetic Oligodeoxyribonucleotides as Mutagens
• Index