Experimental Manipulation of Gene Expression
eBook - ePub

Experimental Manipulation of Gene Expression

  1. 330 pages
  2. English
  3. ePUB (mobile friendly)
  4. Available on iOS & Android
eBook - ePub

Experimental Manipulation of Gene Expression

About this book

Experimental Manipulation of Gene Expression discusses a wide range of host systems in which to clone and express a gene of interest. The aims are for readers to quickly learn the versatility of the systems and obtain an overview of the technology involved in the manipulation of gene expression. Furthermore, it is hoped that the reader will learn enough from the various approaches to be able to develop systems and to arrange for a gene of particular interest to express in a particular system. The book opens with a chapter on the design and construction of a plasmid vector system used to achieve high-level expression of a particular phage regulatory protein normally found in minute amounts in a phage-infected bacterial cell. This is followed by separate chapters on topics such as high-level expression vectors that utilize efficient Escherichia coli lipoprotein promoter as well as various other portions of the lipoprotein gene Ipp; DNA cloning systems for streptomycetes; and the design and application of vectors for high-level, inducible synthesis of the product of a cloned gene in yeast.

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Yes, you can access Experimental Manipulation of Gene Expression by Masayori Inouye in PDF and/or ePUB format, as well as other popular books in Biological Sciences & Biophysics. We have over one million books available in our catalogue for you to explore.

Information

Publisher
Academic Press
Year
2014
Print ISBN
9780123723802
eBook ISBN
9781483273976
Topic
Biological Sciences
Subtopic
Biophysics
Index
Biological Sciences

Table of contents

  1. Cover image
  2. Title page
  3. Table of Contents
  4. Copyright
  5. Contributors
  6. Preface
  7. Chapter 1: Use of Phage λ Regulatory Signals to Obtain Efficient Expression of Genes in Escherichia coli
  8. Chapter 2: Multipurpose Expression Cloning Vehicles in Escherichia coli
  9. Chapter 3: Molecular Cloning in Bacillus subtilis
  10. Chapter 4: Developments in Streptomyces Cloning
  11. Chapter 5: Vectors for High-Level, Inducible Expression of Cloned Genes in Yeast
  12. Chapter 6: Genetic Engineering of Plants by Novel Approaches
  13. Chapter 7: λSV2, a Plasmid Cloning Vector that Can Be Stably Integrated in Escherichia coli
  14. Chapter 8: Construction of Highly Transmissible Mammalian Cloning Vehicles Derived from Murine Retroviruses
  15. Chapter 9: Use of Retrovirus-Derived Vectors to Introduce and Express Genes in Mammalian Cells
  16. Chapter 10: Production of Posttranslationally Modified Proteins in the SV40–Monkey Cell System
  17. Chapter 11: Adenovirus Type 5 Region-E1A Transcriptional Control Sequences
  18. Chapter 12: Expression of Proteins on the Cell Surface Using Mammalian Vectors
  19. Chapter 13: Expression of Human Interferon-γ in Heterologous Systems
  20. Chapter 14: Commercial Production of Recombinant DNA-Derived Products
  21. Appendix 1: Two-Dimensional DNA Electrophoretic Methods Utilizing in Situ Enzymatic Digestions
  22. Appendix 2: Site-Specific Mutagenesis Using Synthetic Oligodeoxyribonucleotides as Mutagens
  23. Index