Nonmammalian Genomic Analysis
eBook - ePub

Nonmammalian Genomic Analysis

A Practical Guide

  1. 353 pages
  2. English
  3. ePUB (mobile friendly)
  4. Available on iOS & Android
eBook - ePub

Nonmammalian Genomic Analysis

A Practical Guide

About this book

Offering detailed protocols for those needing to construct a variety of maps and isolate genes, this unique book is intended to popularize the new techniques of genome analysis derived from the Human Genome Project. The power of these new methods is often most striking when applied to problems outside of human genetics, particularly the nonmammalian systems on which many researchers focus. Many of these organisms are economically important and biologically rich.Nonmammalian Genomic Analysis: A Practical Guide covers the "how to" aspects of preparation, handling, cloning, and analysis of large DNA and the creation of chromosome and genome maps. This lab manual facilitates the transfer of these technologies to small "low tech" environments and allows them to be used by those with no background in genome mapping or large-fragment cloning. Like having a local expert, this collection provides procedures for anyone, anywhere, and allows the replication of others' success.- Includes detailed and clearly-written step-by-step protocols- Evinces expected results and offers trouble shooting advice- Provides techniques appropriate for small laboratories as well as those with limited resources- Covers a broad variety of cloning systems, including single copy vectors- Discusses a diverse range of organisms, from prokaryotes to eukaryotes, from single-celled organisms to highly complex organisms

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Yes, you can access Nonmammalian Genomic Analysis by Bruce Birren,Eric Lai in PDF and/or ePUB format, as well as other popular books in Scienze biologiche & Botanica. We have over one million books available in our catalogue for you to explore.

Information

Publisher
Academic Press
Year
1996
Print ISBN
9780121012854
eBook ISBN
9780080537726
Topic
Scienze biologiche
Subtopic
Botanica

Table of contents

  1. Cover image
  2. Title page
  3. Table of Contents
  4. Copyright
  5. Contributors
  6. Preface
  7. Chapter 1: Introduction to Pulsed-Field Gels and Preparation and Analysis of Large DNA
  8. Chapter 2: Electrophoretic Karyotyping in Fungi
  9. Chapter 3: Isolation and Analysis of High-Molecular-Weight DNA from Plants
  10. Chapter 4: Generating and Using DNA Markers in Plants
  11. Chapter 5: Genome Mapping of Protozoan Parasites by Linking Clones
  12. Chapter 6: Macrorestriction Mapping and Analysis of Bacterial Genomes
  13. Chapter 7: Cosmid Cloning with Small Genomes
  14. Chapter 8: Construction of P1 Artificial Chromosome (PAC) Libraries from Lower Vertebrates
  15. Chapter 9: The Selection of Chromosome-Specific DNA Clones from African Trypanosome Genomic Libraries
  16. Chapter 10: Analysis of the Dictyostelium discoideum Genome
  17. Chapter 11: Integrated Genome Mapping by Hybridization Techniques
  18. Index