Contemporary Enzyme Kinetics and Mechanism

Selected Methods in Enzymology

Daniel L. Purich

• Publisher: Academic Press
• Year: 1983
• Print ISBN:
  9780125680509
• eBook ISBN:
  9781483214221
• Topic: Biological Sciences
• Subtopic: Biochemistry
• Index: Biological Sciences

Selected Methods in Enzymology: Contemporary Enzyme Kinetics and Mechanism provides an introduction to enzyme kinetics and mechanism at an intermediate level. This book covers a variety of topics, including temperature effects in enzyme kinetics, cryoenzymology, substrate inhibition, enol intermediates enzymology, and heavy-atom isotope effects. Organized into 19 chapters, this book begins with an overview of derivation of rate equations as an integral part of the effective usage of kinetics as a tool. This text then examines the practical aspects of initial rate enzyme assay. Other chapters consider the basic procedures used in making decisions concerning kinetic mechanisms from initial-rate data. This book discusses as well the various aspects of both the theoretical background and the applications. The final chapter deals with the importance of achieving proficiency in formulating quantitative relationships describing enzyme behavior. This book is a valuable resource for students and research workers. Enzymologists and chemists will also find this book useful.

1

Derivation of Initial Velocity and Isotope Exchange Rate Equations

CHARLES Y. HUANG

A rate equation for an enzymic reaction is a mathematical expression that depicts the process in terms of rate constants and reactant concentrations. It serves as a link between the experimentally observed kinetic behavior and a plausible model or mechanism. The characteristics of the rate equation permit tests to be designed to verify the mechanism. Conversely, the experimental observations provide clues to what the mechanism may be, hence, what form the rate expression shall take.Derivation of rate equations is an integral part of the effective usage of kinetics as a tool. Novel mechanisms must be described by new equations, and familiar ones often need to be modified to account for minor deviations from the expected pattern. The mathematical manipulations involved in deriving initial veloci...

Table of contents

• Cover image
• Title page
• Table of Contents
• Inside Front Cover
• Copyright
• List of Contributors
• Foreword
• Preface
• Contents of Methods in Enzymology Volumes 63, 64, and 87
• Chapter 1: Derivation of Initial Velocity and Isotope Exchange Rate Equations
• Chapter 2: Practical Considerations in the Design of Initial Velocity Enzyme Rate Assays
• Chapter 3: Plotting Methods for Analyzing Enzyme Rate Data
• Chapter 4: Regression Analysis, Experimental Error, and Statistical Criteria in the Design and Analysis of Experiments for Discrimination Between Rival Kinetic Models
• Chapter 5: Effects of pH on Enzymes
• Chapter 6: Temperature Effects in Enzyme Kinetics
• Chapter 7: Cryoenzymology: The Study of Enzyme Catalysis at Subzero Temperatures
• Chapter 8: Product Inhibition and Abortive Complex Formation
• Chapter 9: Use of Competitive Inhibitors to Study Substrate Binding Order
• Chapter 10: Substrate Inhibition
• Chapter 11: Cooperativity in Enzyme Function: Equilibrium and Kinetic Aspects
• Chapter 12: Application of Affinity Labeling for Studying Structure and Function of Enzymes
• Chapter 13: Criteria for Evaluating the Catalytic Competence of Enzyme–Substrate Covalent Compounds
• Chapter 14: Enzymology of Enol Intermediates
• Chapter 15: Stereochemistry of Enzymic Phosphoryl and Nucleotidyl Transfer
• Chapter 16: Isotope Exchange Methods for Elucidating Enzymic Catalysis
• Chapter 17: The Use of Isotope Effects to Determine Transition-State Structure for Enzymic Reactions
• Chapter 18: Determination of Heavy-Atom Isotope Effects On Enzyme-Catalyzed Reactions
• Chapter 19: Selected Exercises and Problems
• Index