
- 386 pages
- English
- ePUB (mobile friendly)
- Available on iOS & Android
Molecular Methods for Virus Detection
About this book
Molecular diagnostic procedures have been described in a number of recent books and articles. However, these publications have not focused on virus detection, nor have they provided practical protocols for the newer molecular methods.Written by the inventors or principal developers of these technologies, Molecular Methods for Virus Detection provides both reviews of individual methods and instructions for detecting virus nucleic acid sequences in clinical specimens. Each procedure includes quality assurance protocols that are often ignored by other methodology books. Molecular Methods for Virus Detection provides clinically relevant procedures for many of the newer diagnostic methodologies.- Provides state-of-the-art PCR methods for amplification, quantitation, in situ hybridization, and multiplex reactions- Goes beyond PCR with protocols for 3SR, NASBA, LCR, SDA, and LAT- Covers important virus detection methods such as in situ hybridization; Southern, dot, and slot blots; branched chain signal amplification; and chemiluminescence- Includes quality control information crucial in research and clinical laboratories- Most chapters are written by the inventors and principal developers of the methodologies- Includes color plates, 77 figures, and 18 tables
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Table of contents
- Cover image
- Title page
- Table of Contents
- Copyright
- Contributors
- Preface
- Chapter 1: Nucleic Acid Detection Methods
- Chapter 2: Quality Assurance in the Molecular Virology Laboratory
- Chapter 3: Nucleic Acid Blotting Techniques for Virus Detection
- Chapter 4: In Situ Hybridization
- Chapter 5: Antiviral Susceptibility Testing Using DNA-DNA Hybridization
- Chapter 6: Quantification of Viral Nucleic Acids Using Branched DNA Signal Amplification
- Chapter 7: Detection Methods Using Chemiluminescence
- Chapter 8: Detection of Viral Pathogens Using PCR Amplification
- Chapter 9: Quantification of RNA Targets Using the Polymerase Chain Reaction
- Chapter 10: Multiplex Polymerase Chain Reaction
- Chapter 11: PCR in Situ Hybridization
- Chapter 12: Nucleic Acid Sequence-Based Amplification
- Chapter 13: The Self-Sustained Sequence Replication Reaction and Its Application in Clinical Diagnostics and Molecular Biology
- Chapter 14: Ligase Chain Reaction for the Detection of Infectious Agents
- Chapter 15: A Chemiluminescent DNA Probe Test Based on Strand Displacement Amplification
- Chapter 16: Ligation-Activated Transcription Amplification: Amplification and Detection of Human Papillomaviruses
- Index
- Colar Plate