Lectins: Analytical Technologies

Carol L. Nilsson

• Publisher: Elsevier Science
• Year: 2011
• eBook ISBN: 9780080548661
• Topic: Physical Sciences
• Subtopic: Analytic Chemistry

Lectins: Analytical Technologies covers both analytical and biological aspects of lectins (functional carbohydrate (complex sugar) recognition proteins) and provides researchers in the field with a resource containing background information and 'look-up' tables detailing lectin specificity and structures. Also included are methods and practical tips for designing new lectins from existing non-lectin proteins, automated approaches to lectin proteomics and high resolution mass spectrometry techniques. This book will be of interest to both novice and advanced researchers in biomedical, analytical and pharmaceutical fields who are involved in the study of lectin structures or who utilize lectins as analytical tools. The study of lectins and their employment in analytical settings spans a range of fields including: * Crystallography and lectin structure databases* Carbohydrate microarrays for lectin characterization and glycotope identification* Proteomic approaches to the functional identification of bacterial adhesins* Generation of lectins from enzymes* Probing cell-surface lectins with neoglycoconjugates* Reviews up-to-date techniques, including practical hints for laboratory work* Provides overview of lectin e-resources and several color illustrations* Includes a 'look-up' table detailing lectin specificity

Table of contents

• Cover image
• Title page
• Table of Contents
• Preface
• List of Contributors
• Chapter 1: Lectins: Analytical Tools from Nature
• Chapter 2: Crystallography and Lectin Structure Database
• Chapter 3: NMR Investigations of Lectin—Carbohydrate Interactions
• Chapter 4: Applications of Isothermal Titration Calorimetry to Lectin–Carbohydrate Interactions
• Chapter 5: Bioanalytical Studies Based on Lectin–Carbohydrate Interactions Measured by Ellipsometry and Surface Plasmon Resonance Techniques
• Chapter 6: Use of Natural and Synthetic Oligosaccharide, Neoglycolipid and Glycolipid Libraries in Defining Lectins from Pathogens
• Chapter 7: Carbohydrate Microarrays for Lectin Characterization and Glyco-Epitope Identification
• Chapter 8: Glycoproteomics Based on Lectin Affinity Chromatographic Selection of Glycoforms
• Chapter 9: Miniaturized Lectin Affinity Chromatography
• Chapter 10: Frontal Affinity Chromatography: Systematization for Quantitative Interaction Analysis Between Lectins and Glycans
• Chapter 11: Technical Aspects of Glycoprotein Enrichment
• Chapter 12: Proteomic Techniques for Functional Identification of Bacterial Adhesins
• Chapter 13: The Use of Lectins in Bioaffinity MALDI Probes
• Chapter 14: Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry for Lectin Analysis
• Chapter 15: Catalytically Inactive Endoglycosidases as Microbial Diagnostic Reagents: Chitinases and Lysozymes as Fungal and Bacterial Capture/Label Agents
• Chapter 16: Generation of Lectins from Enzymes: Use of Inactive Endosialidase for Polysialic Acid Detection
• Chapter 17: Probing Cell Surface Lectins with Neoglycoconjugates
• Appendix 1: Table of Lectin Affinities
• Appendix 2: List of Abbreviations
• Subject Index