The various cell types have traditionally been recognized and classified according to their appearance in the light microscope following the process of fixing, processing, sectioning, and staining tissues that is known as histology. Classical histology has been augmented by immunohistochemistry (the use of specific antibodies to stain particular molecular species in situ). Immunohistochemistry has allowed the identification of many more cell types than could be visualized by classical histology, particularly in the immune system and among the scattered hormone-secreting cells of the endocrine system.Handbook of Immunohistochemistry and in Situ Hybridization of Human Carcinomas discusses all aspects of immunohistochemistry and in situ hybridization technologies and the important role they play in reaching a cancer diagnosis. It provides step-by-step instructions on the methods of additional molecular technologies such as DNA microarrays, and microdissection, along with the benefits and limitations of each method. The topics of region-specific gene expression, its role in cancer development and the techniques that assist in the understanding of the molecular basis of disease are relevant and necessary in science today, ensuring a wide audience for this book.- The only book available that translates molecular genetics into cancer diagnosis- Provides the readers with tools necessary to perform and optimize sensitive, powerful techniques, including immunohistochemistry and fluorescence in situ hybridization, used in tumor diagnosis- Written by experts in this field, the book provides theoretical considerations as well as practical approaches to carry out effectively these techniques- Offers suggestions, tips, cautions, and guidelines to avoid artifacts and misdiagnosis- Introduces new techniques to detect genes and proteins involved in the initiation and progression of cancer- Covers the latest developments and a wide range of applications to the detection of antigens and single-copy DNA and RNA- Written in a uniform format, each chapter includes Introduction, Materials required, step-by-step detailed Methods, Results, Discussion, and comprehensive up-to-date References
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Yes, you can access Handbook of Immunohistochemistry and in Situ Hybridization of Human Carcinomas by M. A. Hayat in PDF and/or ePUB format, as well as other popular books in Medicine & Immunology. We have over one million books available in our catalogue for you to explore.
Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, University of Pennsylvania Medical Center, 6 Founders Pavilion, 3400 Spruce Street, Philadelphia, PA 19104
N.J. Agnantis (425)
Department of Pathology, Medical School, University of Iannina, 45110 Ioannina, Greece
Debbie Altomare (307)
Human Genetics Program, Fox Chase Cancer Center, 7701 Burholme Avenue, Philadelphia, PA 19111
J.P. Aubert (115)
Service d’Anatomie Pathologique, Hospital Calmette, CHRU-U Lille, Bd J Leclercq 59037, Lille Cedex, France
Sarah S. Bacus (307)
Ventana Medical Systems, Inc./QDL, 610 Oakmont Lane, Westmont, IL 60559
Ana-Maria Bamberger (337)
Department of Gynecopathology, Institute of Pathology, University Hospital Hamburg-Eppendorf, Matinistrassse 52, 20246 Hamburg, Germany
Christoph M. Bamberger (337)
Department of Medicine I, University Hospital Hamburg-Eppendorf, Matinistrassse 52, 20246 Hamburg, Germany
Sushanta K. Bannerjee (409)
Cancer Research Unit, Research Division, V.A. Medical Center, 4801 Linwood Boulevard, Kansas City, MO 64128
Marika Bogdani (371)
Free University of Brussels (VUB), Academic Hospital, Department of Pathology, Laarrbeeklaan 103, Jette, Brussels, 1090, Belgium
Cecilia Bozzetti (267)
Department of Oncology, University Hospital, Via Gransci 14, 43100 Parma, Italy
Elisabeth Brambilla (105, 133)
Laboratoire de Pathologie Cellulaire, CHU Albert Michallon, BP 217, Grenoble, Cedex, 09, France
Marianne Briffod (523)
Service d’Anatomie et de Cytologie Pathologiques, Centre Rene Huguenin, 35 rue Dailly, Saint-Cloud, 92211, France
M.P. Buisine (115)
Service d’Anatomie Pathologique, Hospital Calmette, CHRU-U Lille, Bd J Leclercq 59037, Lille Cedex, France
Yong-Jig Cho (75)
Vanderbilt-Ingram Cancer Center, Department of Cancer Biology, School of Medicine, Vanderbilt University, Nashville, TN
Cynthia Cohen (289)
Immunohistochemistry, Emory University Hospital, 1364 Clifton Road, Atlanta, GA, 30322
M.C. Copin (115)
Service d’Anatomie Pathologique, Hospital Calmette, CHRU-U Lille, Bd J Leclercq 59037, Lille Cedex, France
Nadia Dandachi (279)
Department of Internal Medicine, Division of Oncology, Medical University Hospital Graz, Auenbruggerplatz 15, A-8036 Graz, Austria
L. Devisme (115)
Service d’Anatomie Pathologique, Hospital Calmette, CHRU-U Lille, Bd J Leclercq 59037, Lille Cedex, France
Adhemar Longatto Filho (513)
Universidade do Minho Escola de Ci
ncias de Saude Campus de Gualtar 4710-057, Braga, Portugal
Sylvie Gazzeri (127, 133)
Groupe de Recherche sur le Cancer de Poumon, INSERM U578, Institut Albert Bonniot, 38706 La Tronche, France
Antonio Giordano (223)
Temple University, Sbarro Institute, Biolife Science Building, Suite 333, 1900 North 12th Street, Philadelphia, PA 19122
Vassilis G. Gorgoulis (141)
Molecular Carcinogenesis Group, Department of Histology-Embryology, Medical School, National Kapodistrian University of Athens, Athens, Greece
A. Goussia (425)
Department of Pathology, Medical School, University of Iannina, 45110 Ioannina, Greece
William Grizzle (181)
Department of Nutrition Sciences, Division of Nutritional Biochemistry and Molecular Biology, University of Alabama, University Station, Birmingham, AL 35294
Tatyana A. Grushko (385)
Department of Medicine, Section of Hematology/Oncology, University of Chicago, 5841 S...
Table of contents
Cover image
Title page
Table of Contents
Front Matter
Copyright page
Dedication
Authors and Coauthors of Volume 1
Foreword
Preface to Volume 1
Preface to Volumes 2 and 3
Contents of Volumes 2 and 3
Introduction to Volumes 2 and 3
Prologue
Selected Definitions
Classification Scheme of Human Cancers
Lung and Breast Carcinomas
Comparison of Immunohistochemistry, in situ Hybridization, Fluorescence in situ Hybridization, and Chromogenic in situ Hybridization
Comparison of Chromogenic in situ Hybridization, Fluorescence in situ Hybridization, and Immunohistochemistry
Target and Signal Amplification to Increase the Sensitivity of in situ Hybridization
Polymerase Chain Reaction Technology
DNA Microarrays Technology
Tissue Microarrays and Their Modifications in High-Throughput Analysis of Clinical Specimens
Gene Expression Profiling Using Microdissection in Cancer Tissues
Differential Display of Gene Expression in Human Carcinomas
Serial Analysis of Gene Expression in Human Diseases
Lung Carcinoma: An Introduction
Histopathological Classification Phenotype and Molecular Pathology of Lung Tumors
Immunohistochemistry and in situ Hybridization of Mucin in Lung Carcinoma
Immunohistochemical Expression of MDM2 in Lung Carcinoma
Immunohistochemical Expression of E2F1 and p14ARF in Lung Carcinoma
Role of Immunohistochemical Expression of Beta-Catenin in Lung Carcinoma
Immunohistochemistry of Laminin-5 in Lung Carcinoma
Role of Immunohistochemical Expression of Caveolin-1 in Lung Carcinoma
Role of Thyroid Transcription Factor-1 in Pulmonary Adenocarcinoma
Role of Global Methylation of DNA in Lung Carcinoma
Immunohistochemical and Molecular Pathology of Angiogenesis in Primary Lung Adenocarcinoma
Immunohistochemistry of Human Leukocyte Antigen Expression in Lung Carcinoma
Immunohistochemistry and in situ Hybridization of Telomerase Expression in Lung Carcinoma
Use of Fluorescence in situ Hybridization in Detecting Lung Cancer Cells
Immunohistochemistry of BCL-2 Gene Expression in Lung Carcinoma
Breast Carcinoma: An Introduction
Expression of Vascular Endothelial Growth Factor Receptor-2/Flk-1/ KDR in Breast Carcinoma
HER-2/neu Gene Amplification and Protein Overexpression in Breast Carcinoma: Immunohistochemistry and Fluorescence in situ Hybridization
HER-2/neu Amplification Detected by Fluorescence in situ Hybridization in Cytological Samples from Breast Cancer
Detection of HER-2 Oncogene in Human Breast Carcinoma Using Chromogenic in situ Hybridization
Immunohistochemical Evaluation of Sentinel Lymph Nodes in Breast Carcinoma
CD10 Expression in Normal Breast and Breast Cancer Tissues
Role of Immunohistochemical Expression of AKT Protein in Breast Carcinoma
Expression of Extracellular Matrix Proteins in Breast Cancer
Immunohistochemistry of Adhesion Molecule CEACAM1 Expression in Breast Carcinoma
Role of Cadherins in Breast Cancer
Immunohistochemical Expression of Erythropoietin and Erythropoietin Receptor in Breast Carcinoma
Loss of BRCA1Gene Expression in Breast Carcinoma
Role of Immunohistochemical Detection of BRCA1 in Breast Cancer
Fluorescence in situ Hybridization of BRCA1Gene in Breast Carcinoma
Immunohistochemistry of c-myc Expression in Breast Carcinoma
Immunohistochemical Localization of Neuropilin-1 in Human Breast Carcinoma
Role of Epidermal Growth Factor Receptor in Breast Carcinoma
Alterations of the Cell Cycle Regulating Proteins in Invasive Breast Cancer
Immunohistochemistry of Estrogen Receptor Expression in Breast Carcinoma
Immunofluorescence and Immunohistochemical Localization of Progesterone Receptors in Breast Carcinoma
Immunohistochemical Expression of Cytosolic Thymidine Kinase in Patients with Breast Carcinoma
Immunohistochemical Detection of Melanoma Antigen E (MAGE) Expression in Breast Carcinoma
Male Breast Carcinoma: Role of Immunohistochemical Expression of Receptors in Male Breast Carcinoma
Detection of Glycoconjugates in Breast Cancer Cell Lines: Confocal Fluorescence Microscopy
Expression of the ETV6-NTRK3 Gene Fusion in Human Secretory Breast Carcinoma
The Role of CA6 Protein Expression in Breast Carcinoma
Immunocytochemistry of Effusions
Immunohistochemistry of Needle Cytopunctures of Breast Carcinomas
