RNA and Protein Synthesis
About this book
RNA and Protein Synthesis is a compendium of articles dealing with the assay, characterization, isolation, or purification of various organelles, enzymes, nucleic acids, translational factors, and other components or reactions involved in protein synthesis. One paper describes the preparatory scale methods for the reversed-phase chromatography systems for transfer ribonucleic acids. Another paper discusses the determination of adenosine- and aminoacyl adenosine-terminated sRNA chains by ion-exclusion chromatography. One paper notes that the problems involved in preparing acetylaminoacyl-tRNA are similar to those found in peptidyl-tRNA synthesis, in particular, to the lability of the ester bond between the amino acid and the tRNA. Another paper explains a new method that will attach fluorescent dyes to cytidine residues in tRNA; it also notes the possible use of N-hydroxysuccinimide esters of dansylglycine and N-methylanthranilic acid in the described method. One paper explains the use of membrane filtration in the determination of apparent association constants for ribosomal protein-RNS complex formation. This collection is valuable to bio-chemists, cellular biologists, micro-biologists, developmental biologists, and investigators working with enzymes.
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Information
Table of contents
- Front Cover
- RNA and Protein Synthesis
- Copyright Page
- Table of Contents
- List of Contributors
- Foreword
- Preface
- Section I: Transfer RNA
- Section II: Aminoacyl-tRNA Synthetases
- Section III: Polypeptide Chain Initiation
- Section IV: Polypeptide Chain Elongation
- Section V: Polypeptide Chain Termination
- Section VI: Ribosomes
- Index
