
- 190 pages
- English
- PDF
- Available on iOS & Android
About this book
Plant Tissue Culture Techniques and Experiments is a manual that contains laboratory exercises about the demonstration of the methods and different plant materials used in plant tissue culture. It provides an overview on the plant cell culture techniques and plant material options in selecting the explant source. This book starts by discussing the proper setup of a tissue culture laboratory and the selection of the culture medium. It then explains the determination of an explant which is the ultimate goal of the cell culture project. The explant is a piece of plant tissue that is used in tissue culture. Furthermore, the book discusses topics about callus induction, regeneration and morphogenesis process, and haploid plants from anther and pollen culture. The meristem culture for virus-free plants and in vitro propagation for commercial propagation of ornamentals are also explained in this manual. The book also provides topics and exercises on the protoplast isolation and fusion and agrobacterium-mediated transformation of plants. This manual is intended for college students, both graduate and undergraduate, who study chemistry, plant anatomy, and plant physiology.
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Information
Table of contents
- Front Cover
- Plant Tissue Culture: Techniques and Experiments
- Copyright Page
- Table of Contents
- Dedication
- Preface
- Acknowledgments
- Scheduling and Interrelationships of Exercises
- Chapter 1. Setup of a Tissue Culture Laboratory
- Chapter 2. Media Components and Preparation
- Chapter 3. Explant Preparation
- Chapter 4. Callus Induction
- Chapter 5. Regeneration and Morphogenesis
- Chapter 6. Haploid Plants from Anther Culture
- Chapter 7. Embryo Rescue
- Chapter 8. Meristem Culture for Virus-Free Plants
- Chapter 9. In Vitro Propagation for Commercial Production of Ornamentals
- Chapter 10. Protoplast Isolation and Fusion
- Chapter 11. Agrobacterium-Mediated Transformation of Plants
- Chapter 12. Useful Measurements
- Chapter 13. Review of Solution Preparation
- Chapter 14. List of Suppliers
- Chapter 15. Common Plant Tissue Culture Terms
- Index