Laboratory Procedures and Their Applications
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Laboratory Procedures and Their Applications

  1. 858 pages
  2. English
  3. PDF
  4. Available on iOS & Android
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Laboratory Procedures and Their Applications

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Table of contents

  1. Front Cover
  2. Laboratory Procedures and Their Applications
  3. Copyright Page
  4. Table of Contents
  5. Dedication
  6. Contributors
  7. General Preface
  8. Preface to Volume 1
  9. Chapter 1. Organization of a Plant Tissue Culture Laboratory
  10. Chapter 2. Educational Services for Plant Tissue Culture
  11. Chapter 3. Plant Cell Cultures: Nutrition and Media
  12. Chapter 4. Callus Culture: Induction and Maintenance
  13. Chapter 5. Induction and Maintenance of Embryogenic Callus Cultures of Gramineae
  14. Chapter 6. Clonal Propagation: Shoot Cultures
  15. Chapter 7. Clonal Propagation: Adventitious Buds
  16. Chapter 8. Clonal Propagation: Orchids
  17. Chapter 9. Clonal Propagation: Somatic Embryos of Citrus
  18. Chapter 10. Clonal Propagation: Palms
  19. Chapter 11. Clonal Propagation: Gymnosperms
  20. Chapter 12. Culture Methods for Bryophytes
  21. Chapter 13. Culture of Shoot Meristems: Pea
  22. Chapter 14. Culture of Shoot Meristems: Fruit Plants
  23. Chapter 15. The Acclimatization of Micropropagated Plants
  24. Chapter 16. Induction and Maintenance of Cell Suspension Cultures
  25. Chapter 17. Induction, Maintenance, and Manipulation of Development in Embryogenic Cell Suspension Cultures
  26. Chapter 18. Isolation and Maintenance of Embryogenic Cell Suspension Cultures of Gramineae
  27. Chapter 19. Fractionation of Cultured Cells
  28. Chapter 20. Large-Scale Cultures of Cells in Suspension
  29. Chapter 21. Synchronization of Suspension Culture Cells
  30. Chapter 22. Photoautotrophic Cell Cultures
  31. Chapter 23. Quantitative Plating Technique
  32. Chapter 24. The Feeder Layer Technique
  33. Chapter 25. Culture of Isolated Mesophyll Cells
  34. Chapter 26. The Multiple-Drop-Array (MDA) Screening Technique
  35. Chapter 27. Culture of Ovaries
  36. Chapter 28. Culture of Ovules
  37. Chapter 29. Culture of Cotton Ovules
  38. Chapter 30. Culture of Embryos
  39. Chapter 31. Culture of Endosperm
  40. Chapter 32. In Vitro Pollination and Fertilization
  41. Chapter 33. In Vitro Pollination, Fertilization, and Development of Maize Kernels
  42. Chapter 34. Anther Culture of Nicotiana tabacum
  43. Chapter 35. Anther Culture of Solanum tuberosum
  44. Chapter 36. Anther Culture of Brassica
  45. Chapter 37. Anther Culture of Cereals and Grasses
  46. Chapter 38. Isolation and Culture of Protoplasts:Tobacco
  47. Chapter 39. Isolation and Culture of Protoplasts: Petunia
  48. Chapter 40. Isolation and Culture of Protoplasts: Datura
  49. Chapter 41. Isolation and Culture of Protoplasts: Brassica
  50. Chapter 42. Isolation and Culture of Protoplasts: Tomato
  51. Chapter 43. Isolation, Culture, and Regeneration of Potato Leaf Protoplasts from Plants Preconditioned in Vitro
  52. Chapter 44. Isolation and Culture of Protoplasts from Carrot Cell Suspension Cultures
  53. Chapter 45. Isolation and Culture of Embryogenic Protoplasts of Cereals and Grasses
  54. Chapter 46. Mechanical Isolation and Single-Cell Culture of Isolated Protoplasts and Somatic Hybrid Cells
  55. Chapter 47. Fusion of Protoplasts by Polyethylene Glycol (PEG)
  56. Chapter 48. Fusion of Protoplasts by Dextran and Electrical Stimulus
  57. Chapter 49. Inactivation of Protoplasts before Fusion to Facilitate Selective Recovery of Fusion-Derived Clones
  58. Chapter 50. Selection of Somatic Hybrid Cells by Fluorescence-Activated Cell Sorting
  59. Chapter 51. Enucleation of Protoplasts: Preparation of Cytoplasts and Miniprotoplasts
  60. Chapter 52. Isolation of Organelles: Nuclei
  61. Chapter 53. Isolation of Organelles: Chromosomes
  62. Chapter 54. Isolation of Organelles: Chloroplasts
  63. Chapter 55. Liposome Preparation and Incubation with Plant Protoplasts
  64. Chapter 56. Inoculation of Protoplasts with Plant Viruses
  65. Chapter 57. Uptake of Organelles
  66. Chapter 58. Transformation of Tobacco Cells by Coculture with Agrobacterium tumefaciens
  67. Chapter 59. Ti-Plasmid DNA Uptake and Expression by Protoplasts of Nicotiana tabacum
  68. Chapter 60. Immobilization of Cultured Plant Cells and Protoplasts
  69. Chapter 61. Mutagenesis of Cultured Cells
  70. Chapter 62. Cell Culture Procedures for Mutant Selection and Characterization in Nicotiana plumbaginifolia
  71. Chapter 63. Induction, Selection, and Characterization of Mutants in Carrot Cell Cultures
  72. Chapter 64. Induction, Selection, and Characterization of Mutants in Maize Cell Cultures
  73. Chapter 65. Elimination of Viruses
  74. Chapter 66. Cocultures of Plant and Bacterial Cells
  75. Chapter 67. Isolation and Bioassay of Fungal Phytotoxins
  76. Chapter 68. Freeze Preservation of Cells
  77. Chapter 69. Freeze Preservation of Meristems
  78. Chapter 70. Selection of Cell Lines for High Yields of Secondary Metabolites
  79. Chapter 71. Isolation and Analysis of Terpenoids
  80. Chapter 72. Isolation and Analysis of Alkaloids
  81. Chapter 73. Protein Extraction and Analysis
  82. Chapter 74. Isolation and Analysis of Plant Growth Regulators
  83. Chapter 75. Plastic Embedding for Light Microscopy
  84. Chapter 76. Histological and Histochemical Staining Procedures
  85. Chapter 77. Staining and Nuclear Cytology of Cultured Cells
  86. Chapter 78. Chromosome Analysis
  87. Chapter 79. Preparation of Cultured Cells and Tissues for Transmission Electron Microscopy
  88. Chapter 80. Preparation of Cultured Tissues for Scanning Electron Microscopy
  89. Chapter 81. Microspectrophotometric Analysis
  90. Chapter 82. Cell Cycle Analysis by Conventional Methods
  91. Chapter 83. Flow Cytometric Analysis of the Cell Cycle
  92. Chapter 84. Autoradiography
  93. Chapter 85. Immunofluorescence Techniques for Studies of Plant Microtubules
  94. Index

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