Partitioning In Aqueous Two – Phase System
eBook - PDF

Partitioning In Aqueous Two – Phase System

Theory, Methods, Uses, and Applications To Biotechnology

  1. 730 pages
  2. English
  3. PDF
  4. Available on iOS & Android
eBook - PDF

Partitioning In Aqueous Two – Phase System

Theory, Methods, Uses, and Applications To Biotechnology

About this book

Partitioning in Aqueous Two-Phase Systems: Theory, Methods, Uses, and Applications to Biotechnology is a collection of papers that discusses the applications of aqueous two-phase systems to problems of separation and extraction of macromolecules, organelles, and cells. Papers focus on the theoretical basis and the practical details of the procedures used. Some of the papers describe in one or a few steps how two components can be separated by the investigator manipulating their partitions so that one component is in one phase and the other component is in the other phase or at the interface. Investigators can also avail of developed batch extractions for plant organelles, cell membranes, nucleic acids, and proteins. The book cites as an example the partitioning of right-side-out and inside-out vesicles (obtained from fragments of thylakoid membranes) to the top and bottom phases, respectively, of a Dx-PEG system. Other papers describe the use of the countercurrent distribution when single extraction steps are not sufficient to produce a separation in materials that do not differ greatly in their partitioning behavior. The collection can prove valuable for bio-chemists, cellular biologists, micro-biologists, and developmental biologists.

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Yes, you can access Partitioning In Aqueous Two – Phase System by Harry Walter in PDF and/or ePUB format, as well as other popular books in Biological Sciences & Biochemistry. We have over one million books available in our catalogue for you to explore.

Table of contents

  1. Front Cover
  2. Partitioning in Aqueous Two-Phase Systems: Theory, Methods, Uses, and Applications to Biotechnology
  3. Copyright Page
  4. Table of Contents
  5. Contributors
  6. Preface
  7. Abbreviations and Conventions
  8. Chapter 1. History of Aqueous Polymer Two-Phase Partition
  9. Chapter 2. Theoretical Aspects of Partitioning
  10. Chapter 3. Preparation of Phase Systems and Measurement of Their Physicochemical Properties
  11. Chapter 4. Thin-Layer Countercurrent Distribution and Apparatus
  12. Chapter 5. Other Types of Countercurrent Distribution Apparatus and Continuous Flow Chromatography Techniques
  13. Chapter 6. Partitioning of Proteins
  14. Chapter 7. Partitioning of Nucleic Acids
  15. Chapter 8. Interacting Systems and Binding Studied by Partitioning
  16. Chapter 9. Steroid Receptors and Steroid-Binding Plasma Proteins Studied by Partitioning
  17. Chapter 10. Surface Properties of Cells Reflected by Partitioning: Red Blood Cells as a Model
  18. Chapter 11. Separation and Subfractionation of Selected Mammalian Cell Populations
  19. Chapter 12. Partitioning of Bacteria, Virus, and Phage
  20. Chapter 13. Partitioning of Animal Membranes and Organelles
  21. Chapter 14. Partitioning of Plant Cells, Cell Walls, Membranes, and Organelles
  22. Chapter 15. Applications of Phase Partitioning in Biotechnology
  23. Chapter 16. Polymer-Ligands Used in Affinity Partitioning and Their Synthesis
  24. Chapter 17. Partitioning: A Comprehensive Bibliography
  25. Index