Transgenesis and Targeted Mutagenesis in Immunology

Horst Bluethmann, Pamela Ohashi, Horst Bluethmann, Pamela Ohashi

• Publisher: Academic Press
• Year: 2014
• Print ISBN:
  9780121057602
• eBook ISBN:
  9781483296494
• Topic: Medicine
• Subtopic: Immunology

Investigations into the field of immunology are rapidly expanding with the use of genetically altered mice at the embryonic stage. This breakthrough laboratory guide provides a complete study of transgenesis and targeted mutagenesis in laboratory mice that will be valued by researchers looking for fresh observations and interpretations when designing future experiments.Special Features Include:Contributions of two Nobel Prize winnersAddresses the use of mouse models in studying the immune systemTargets gene distribution in embryonic stem cells and their introduction into blastocyte mice modelsAnalyzes the in vivo functional loss of embryonic cellsA practical, useful guidebook for individual researchers, laboratories, and libraries

Table of contents

• Front Cover
• Transgenesis and Targeted Mutagenesis in Immunology
• Copyright Page
• Table of Contents
• Contributors
• Preface
• Chapter 1. Transgenic Mouse Models to Study VDJ Recombination
• Chapter 2. Lymphocyte Development and Function in T Cell Receptor and RAG-1Mutant Mice
• Chapter 3. RAG-2-Deficient Blastocyst Complementation
• Chapter 4. Role of CD4 and CD8 in Determining Cell Fate in the Thymus
• Chapter 5. T Cell Development in CD4, CD8, and p56ICK Gene-Targeted Mice
• Chapter 6. T Cell Tolerance in T Cell Receptor Transgenic Ipr/lpr Mice
• Chapter 7. Thymocyte Selection and Peripheral Tolerance Using the Lymphocytic Choriomeningitis Virus as a Model Antigen
• Chapter 8. Peripheral T Cell Tolerance: Distinct Levels and Multistep Mechanisms
• Chapter 9. Peripheral Tolerance and CD4+ T Cells
• Chapter 10. Studies of Tolerance, Inflammation, and Autoimmunity inTransgenic Mice
• Chapter 11. Immunological Studies Utilizing Cytokine Transgenic Mice
• Chapter 12. Effect of MHC Class II Encoding Transgenes on Autoimmunity in Nonobese Diabetic Mice
• Chapter 13. Development and Function of the Immune System in Mice with Targeted Disruption of the Interleukin 2 Gene
• Chapter 14. Immune Responses in Interleukin 4-Deficient Mice
• Chapter 15. Manipulation of Transgene-Encoded Self-Antigens to Explore Mechanisms of B Cell Tolerance
• Chapter 16. Transgenic Mouse Model for Peripheral B Cell Tolerance
• Chapter 17. Immunobiology of MHC Class l-Deficient Mice
• Chapter 18. MHC Class II Mutant Mice
• Index