Histochemistry

An Explanatory Outline of Histochemistry and Biophysical Staining

Richard W. Horobin

• Publisher: Butterworth-Heinemann
• Year: 2014
• Print ISBN: 9783437107009
• eBook ISBN: 9781483164687
• Topic: Biowissenschaften
• Subtopic: Zellbiologie

Histochemistry: An Explanatory Outline of Histochemistry and Biophysical Staining describes the histochemical staining of cells and tissues as a major tool applied in biological and medical investigations, both in basic research and in practical applications such as clinical diagnosis. The book may be considered as a guide to understanding the scientific basis of staining procedures and alternate actions to take when common methods do not proceed as expected. The first chapter gives general theoretical ideas from which most part of the book is largely organized around. As the book considers the arts and crafts making up the practice of histochemistry and biological staining, emphasis is given to the common physicochemical aspects of the technically diverse methodologies involved. Hence, the author has drawn ideas and information from physicochemically and biochemically related fields, such as chromatography, pharmacology, photography, tanning, and textile dyeing. The bulk of the book is structured around groups of practical procedures, such as fixation, staining with dyestuffs, metal impregnation, and selective extraction as a test of significance. Lastly, general essays on the usefulness of staining theories and on the problems arising from reagent impurities are given as examples. The text is suitable for students and researchers in the fields of physiology, biochemistry, and pharmacology. Clinical laboratory scientists and those involved in the life sciences and biotechnology will benefit from reading the book.

Table of contents

• Front Cover
• Histochemistry: An Explanatory Outline of Histochemistry and Biophysical Staining
• Copyright Page
• Table of Contents
• Preface
• Chapter 1. An overview of biological staining
• Chapter 2. The preparation of material to be stained
• Chapter 3. Fixation and other loss-limiting procedures
• Chapter 4. Staining methods involving dyeing
• Chapter 5. Reactive staining methods
• Chapter 6. Staining methods involving uptake of metals
• Chapter 7. Enzyme histochemistry
• Chapter 8. Immuno-chemical staining using labelled antibody and related methods
• Chapter 9. Some miscellaneous biological staining methods
• Chapter 10. Peculiarities of staining plastic sections
• Chapter 11. The interpretation and analysis of histochemical observations
• Chapter 12. Supplements
• References
• Subject Index