Methods in Plant Molecular Biology

Mary A. Schuler, Raymond E. Zielinski

• Publisher: Academic Press
• Year: 2012
• Print ISBN:
  9780126323405
• eBook ISBN:
  9780323137652
• Topic: Biological Sciences
• Subtopic: Botany
• Index: Biological Sciences

Methods in Plant Molecular Biology is a lab manual that introduces students to a diversity of molecular techniques needed for experiments with plant cells. Those included have been perfected and are now presented for the first time in a usable and teachable form. Because the manual integrates protein, RNA, and DNA techniques, it will serve students, teachers, and researchers in plant physiology, biophysics, and animal molecular biology who have no previous experience handling recombinant DNA or purified proteins. It can also be used by the established molecular biologist who wishes to utilize the powerful techniques of recombinant DNA to explore the mysteries of the plant kingdom. - Eight basic experiments which can be used collectively or individually cover - Recombinant Cloning and Screening in E. coli; DNA Sequencing - Plant RNA Isolation and in Vitro Translations - Plant DNA Isolations and Genomic DNA Southern AnalysisChloroplast Isolation and Protein SynthesisPlant Tissue Culture and Agrobacterium Transformations - Experiments that have been student tested for three years - Blueprints for setting up gel rigs - Comprehensive course schedule outlining individual procedures to be finished in each lab segment - Course can be tailored to suit the needs of the individual instructor

Table of contents

• Front Cover
• Methods in Plant Molecular Biology
• Copyright Page
• Table of Contents
• Preface
• Laboratory Schedule
• Chapter 1. Restriction Mapping of Plasmid DNA
• Chapter 2. Cloning of Restriction Fragments into pUC Vectors
• Chapter 3A. Preparation of Intact Chloroplasts from Pea
• Chapter 3B. Lowry Assay for Protein Determination
• Chapter 3C. Protein Synthesis by Isolated Pea Chloroplasts
• Chapter 3D. Separation of Thylakoid and Stromal Proteins by SDS-Gel Electrophoresis
• Chapter 4. Isolation of Chloroplast DNA
• Chapter 5A. RNA Isolation from Light- and Dark-Grown Seedlings
• Chapter 5B. Preparation of a Wheat Germ Extract for in Vitro Translation of mRNA
• Chapter 6A. Screening of Recombinant Phage Libraries with Cloned CDNA Probes
• Chapter 6B. Isolation of Phage DNA from Liquid Cultures
• Chapter 7. Dideoxy DNA Sequencing
• Chapter 8. Transformation of Leaf Discs with Agrobacterium
• Appendix I: Glossary
• Appendix II: Gel Electrophoresis Equipment
• Index