Methods in Plant Molecular Biology
eBook - PDF

Methods in Plant Molecular Biology

  1. 171 pages
  2. English
  3. PDF
  4. Available on iOS & Android
eBook - PDF

Methods in Plant Molecular Biology

About this book

Methods in Plant Molecular Biology is a lab manual that introduces students to a diversity of molecular techniques needed for experiments with plant cells. Those included have been perfected and are now presented for the first time in a usable and teachable form. Because the manual integrates protein, RNA, and DNA techniques, it will serve students, teachers, and researchers in plant physiology, biophysics, and animal molecular biology who have no previous experience handling recombinant DNA or purified proteins. It can also be used by the established molecular biologist who wishes to utilize the powerful techniques of recombinant DNA to explore the mysteries of the plant kingdom.- Eight basic experiments which can be used collectively or individually cover- Recombinant Cloning and Screening in E. coli; DNA Sequencing- Plant RNA Isolation and in Vitro Translations- Plant DNA Isolations and Genomic DNA Southern AnalysisChloroplast Isolation and Protein SynthesisPlant Tissue Culture and Agrobacterium Transformations- Experiments that have been student tested for three years- Blueprints for setting up gel rigs- Comprehensive course schedule outlining individual procedures to be finished in each lab segment- Course can be tailored to suit the needs of the individual instructor

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Yes, you can access Methods in Plant Molecular Biology by Mary A. Schuler,Raymond E. Zielinski in PDF and/or ePUB format, as well as other popular books in Biological Sciences & Botany. We have over one million books available in our catalogue for you to explore.

Information

Publisher
Academic Press
Year
2012
Print ISBN
9780126323405
eBook ISBN
9780323137652
Topic
Biological Sciences
Subtopic
Botany
Index
Biological Sciences

Table of contents

  1. Front Cover
  2. Methods in Plant Molecular Biology
  3. Copyright Page
  4. Table of Contents
  5. Preface
  6. Laboratory Schedule
  7. Chapter 1. Restriction Mapping of Plasmid DNA
  8. Chapter 2. Cloning of Restriction Fragments into pUC Vectors
  9. Chapter 3A. Preparation of Intact Chloroplasts from Pea
  10. Chapter 3B. Lowry Assay for Protein Determination
  11. Chapter 3C. Protein Synthesis by Isolated Pea Chloroplasts
  12. Chapter 3D. Separation of Thylakoid and Stromal Proteins by SDS-Gel Electrophoresis
  13. Chapter 4. Isolation of Chloroplast DNA
  14. Chapter 5A. RNA Isolation from Light- and Dark-Grown Seedlings
  15. Chapter 5B. Preparation of a Wheat Germ Extract for in Vitro Translation of mRNA
  16. Chapter 6A. Screening of Recombinant Phage Libraries with Cloned CDNA Probes
  17. Chapter 6B. Isolation of Phage DNA from Liquid Cultures
  18. Chapter 7. Dideoxy DNA Sequencing
  19. Chapter 8. Transformation of Leaf Discs with Agrobacterium
  20. Appendix I: Glossary
  21. Appendix II: Gel Electrophoresis Equipment
  22. Index