Colloidal Gold
eBook - PDF

Colloidal Gold

Principles, Methods, and Applications

,
  1. 517 pages
  2. English
  3. PDF
  4. Available on iOS & Android
eBook - PDF

Colloidal Gold

Principles, Methods, and Applications

,

About this book

Since its introduction in 1971, the development and application of colloidal gold as a marker in electron microscopy has been phenomenal. Colloidal gold has become the method of choice in immunocytochemistry and many areas of cell biology. This universal method is applicable to most microscopical systems including optical microscopy; scanning, transmission, and high voltage electron microscopy; photoelectron, photon, fluorescent darkfield, and epipolarization microscopy. Colloidal gold allows high and low resolution studies, enzyme and nucleic acid labeling, study of dynamic cellular processes, and virus detection. This book is among the first available to cover the principles and methodology of colloidal gold in microscopy. - Methods are described step by step, to enable researchers to learn these complex procedures solely by reference to these books - Problems and limitations of techniques are discussed - Guides users to avoid problems and choose the correct procedures for specific applications - Contributors are eminent authorities in their fields

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Table of contents

  1. Front Cover
  2. Colloidal Gold: Principles, Methods, and Applications
  3. Copyright Page
  4. Table of Contents
  5. List of Contributors
  6. Preface
  7. Contents of Other Volumes
  8. Chapter 1. Molecular Interactions between Colloidal Gold, Proteins, and Living Cells
  9. Chapter 2. Protein A—Gold: Nonspecific Binding and Cross-Contamination
  10. Chapter 3. Role of Tissue Processing in Colloidal Gold Methods
  11. Chapter 4. LR White Embedding Medium for Colloidal Gold Methods
  12. Chapter 5. Lowicryls and Low-Temperature Embedding for Colloidal Gold Methods
  13. Chapter 6. The Enzyme—Gold Cytochemical Approach: A Review
  14. Chapter 7. Preparation and Application of Lipoprotein—Gold Complex
  15. Chapter 8. Preparation and Application of Albumin—Gold Complex
  16. Chapter 9. Label-Fracture Cytochemistry
  17. Chapter 10. Colloidal Gold Conjugates for Retrograde Neuronal Tracing
  18. Chapter 11. Colloidal Gold Labeling of Microtubules in Cleaved Whole Mounts of Cells
  19. Chapter 12. Colloidal Gold: Immunonegative Staining Method
  20. Chapter 13. Immunogold Labeling of Viruses in Situ
  21. Chapter 14. Study of Exocytosis with Colloidal Gold and Other Methods
  22. Chapter 15. Colloidal Gold Labeling of Acrylic Resin-Embedded Plant Tissues
  23. Chapter 16. Preembedding Immunogold Staining of Cell Surface-Associated Antigens Performed on Suspended Cells and Tissue Sections
  24. Chapter 17. Colloidal Gold in High-Voltage Electron Microscopy—Ruthenium Red Method and Whole-Cell Mount
  25. Chapter 18. Correlative Light and Electron Microscopic Immunocytochemistry on Reembedded Resin Sections with Colloidal Gold
  26. Chapter 19. Streptavidin—Gold Labeling for Ultrastructural in Situ Nucleic Acid Hybridization
  27. Chapter 20. Detection of Proteins with Colloidal Gold
  28. Chapter 21. Undecagold—Antibody Method
  29. Chapter 22. Immunogold Labeling for the Single-Laser FACS Analysis of Triple Antibody-Binding Cells
  30. Chapter 23. Silver-Enhanced Colloidal Gold for the Detection of Leukocyte Cell Surface Antigens in Dark-Field and Epipolarization Microscopy
  31. Index

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